MOSC2 Polyklonaler Antikörper

MOSC2 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 24782-1-AP

Synonyme

MOSC domain-containing protein 2, Molybdenum cofactor sulfurase C-terminal domain-containing protein 2, Moco sulfurase C-terminal domain-containing protein 2, Mitochondrial amidoxime reducing component 2, mARC2

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  WB analysis using 24782-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24782-1-AP (MOSC2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 24782-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24782-1-AP (MOSC2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse liver using 24782-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 24782-1-AP (MOSC2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 24782-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 24782-1-AP (MOSC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 24782-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24782-1-AP (MOSC2 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver cancer using 24782-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24782-1-AP (MOSC2 Antibody) at dilution of 1:200 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausnierengewebe, Mauslebergewebe, Rattennierengewebe, Rattenlebergewebe
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, Mauslebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

24782-1-AP bindet in WB, IHC, ELISA MOSC2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MOSC2 fusion protein Ag20694
• Vollständiger Name: MOCO sulphurase C-terminal domain containing 2
• Berechnetes Molekulargewicht: 335 aa, 38 kDa
• Beobachtetes Molekulargewicht: 35-38 kDa
• GenBank-Zugangsnummer: BC011973
• Gene symbol: MOSC2
• Gene ID (NCBI): 54996
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MOSC domain-containing protein 2 (also known as MOSC2), also known as MARC2, is a component of prodrug-converting system, reduces a multitude of N-hydroxylated prodrugs particularly amidoximes, leading to increased drug bioavailability. Also, MOSC2 may be involved in mitochondrial N(omega)-hydroxy-L-arginine (NOHA) reduction, regulating endogenou1s nitric oxide levels and biosynthesis. The reductase activity is regulated under adipogenic conditions, and down-regulation of the terminal component MOSC2 resulted in decreased lipid synthesis, suggesting a possible physiological role of this enzyme system and its component MOSC2 in lipogenesis(PMID: 22203676).

Publikationen

Species	Application	Title
human	WB	Cancers (Basel); RNA Sequencing of Collecting Duct Renal Cell Carcinoma Suggests an Interaction between miRNA and Target Genes and a Predominance of Deregulated Solute Carrier Genes.; Authors - Sven Wach
		PLoS Genet; Divergent role of Mitochondrial Amidoxime Reducing Component 1 (MARC1) in human and mouse; Authors - Eriks Smagris