MOSPD2 Rekombinanter Antikörper

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• KD/KO Validated

MOSPD2 Rekombinant Antikörper für WB, IHC, FC (Intra), Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Ratte

Anwendung

WB, IHC, FC (Intra), Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

240282C11

Kat-Nr. : 83398-1-PBS

Synonyme

240282C11, Motile sperm domain-containing protein 2

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  WB analysis using 83398-1-RR (same clone as 83398-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83398-1-RR (MOSPD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83398-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 83398-1-RR (same clone as 83398-1-PBS)

  WB result of MOSPD2 antibody (83398-1-RR; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MOSPD2 transfected HeLa cells. This data was developed using the same antibody clone with 83398-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 83398-1-RR (same clone as 83398-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83398-1-RR (MOSPD2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83398-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse placenta using 83398-1-RR (same clone as 83398-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse placenta tissue slide using 83398-1-RR (MOSPD2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83398-1-PBS in a different storage buffer formulation.

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  FC experiment of A431 using 83398-1-RR (same clone as 83398-1-PBS)

  1x10^6 A431 cells were intracellularly stained with 0.25 ug MOSPD2 Recombinant antibody (83398-1-RR, Clone:240282C11) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83398-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00419-2

  Cytometric bead array standard curve of MP00419-2, MOSPD2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83398-1-PBS. Detection antibody: 83398-2-PBS. Standard: Ag22206. Range: 0.78-100 ng/mL.

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  Cytometric bead array standard curve of MP00419-3

  Cytometric bead array standard curve of MP00419-3, MOSPD2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83398-3-PBS. Detection antibody: 83398-1-PBS. Standard: Ag22206. Range: 0.78-100 ng/mL.

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  Affinity and Kinetic Characterization of 83398-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83398-1 -RR against Human MOSPD2 were performed. The affinity constant is 0.245 nM.

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Geprüfte Anwendungen

Produktinformation

83398-1-PBS bindet in WB, IHC, FC (Intra), Cytometric bead array, Indirect ELISA MOSPD2 und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: MOSPD2 fusion protein Ag22206
• Vollständiger Name: motile sperm domain containing 2
• Berechnetes Molekulargewicht: 518 aa, 60 kDa
• Beobachtetes Molekulargewicht: 59 kDa
• GenBank-Zugangsnummer: BC030641
• Gene symbol: MOSPD2
• Gene ID (NCBI): 158747
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MOSPD2 is a 518-amino acid protein containing an N-terminal CRAL/TRIO domain, a major sperm protein (MSP) domain, and a potential C-terminal transmembrane domain. MOSPD2 is a member of the vesicle-associated membrane protein-associated protein (VAP) family and one of the endoplasmic reticulum (ER) receptors that function as tether proteins to bridge ER and other organelles to build contacts(PMID: 29858488, 35389430).