MOV10 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

MOV10 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA

Konjugation

Unkonjugiert

Publikationen(28)

Kat-Nr. : 10370-1-AP

Synonyme

Armitage homolog, EC:3.6.4.13, fSAP113, gb110, Helicase MOV-10

---

• 

  WB analysis using 10370-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10370-1-AP (MOV10 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 10370-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10370-1-AP (MOV10 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 10370-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 10370-1-AP (MOV10 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 10370-1-AP

  IP result of anti-MOV10 (IP:10370-1-AP, 4ug; Detection:10370-1-AP 1:5000) with HepG2 cells lysate 1480 ug.

• 

  IHC staining of human prostate hyperplasia using 10370-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 10370-1-AP (MOV10 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human prostate hyperplasia using 10370-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 10370-1-AP (MOV10 antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of HepG2 using 10370-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MOV10 antibody (10370-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  IF Staining of MCF-7 using 10370-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using MOV10 antibody (10370-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, humanes Lebergewebe, MCF-7-Zellen, Mauslebergewebe, Rattenlebergewebe
• Erfolgreiche IP: HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Prostatahyperplasie-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen, MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 5 publications below
• WB: See 23 publications below
• IHC: See 1 publications below
• IF: See 8 publications below
• IP: See 4 publications below
• CoIP: See 1 publications below
• RIP: See 1 publications below

Produktinformation

10370-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA MOV10 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MOV10 fusion protein Ag0384
• Vollständiger Name: Mov10, Moloney leukemia virus 10, homolog (mouse)
• Berechnetes Molekulargewicht: 114 kDa
• Beobachtetes Molekulargewicht: 110-115 kDa
• GenBank-Zugangsnummer: BC002548
• Gene symbol: MOV10
• Gene ID (NCBI): 4343
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MOV10, also named as KIAA1631, belongs to the DNA2/NAM7 helicase family and SDE3 subfamily. It is required for RNA-mediated gene silencing by the RNA-induced silencing complex (RISC). Human MOV10 may regulate a wide range of RNA viruses and could also control the retrotransposition of endogenous retroelements in mammals(PMID:22727223). MOV10 has a broad antiretroviral activity that can target a wide range of retroviruses, and it could be actively involved in host defense against retroviral infection. MOV10 can potently inhibit HIV-1 replication at multiple stages(PMID:20215113). It is involved in the progression of telomerase-catalyzing reaction via the interaction of telomerase protein and telomere DNA.

Publikationen

Species	Application	Title
human	WB	Neuron; A coordinated local translational control point at the synapse involving relief from silencing and MOV10 degradation.; Authors - Banerjee Sourav S
		Mol Cell; Multivalent Proteins Rapidly and Reversibly Phase-Separate upon Osmotic Cell Volume Change.; Authors - Ameya P Jalihal
human	WB	Mol Cell; MOV10 Is a 5' to 3' RNA helicase contributing to UPF1 mRNA target degradation by translocation along 3' UTRs.; Authors - Lea H Gregersen ; KD Validated
human	WB,IP	Nat Struct Mol Biol; Capped small RNAs and MOV10 in human hepatitis delta virus replication.; Authors - Haussecker Dirk D ; KD Validated
human	WB,IF	PLoS Genet; MOV10 RNA helicase is a potent inhibitor of retrotransposition in cells.; Authors - Goodier John L JL
human	IF	PLoS Pathog; APOBEC3G and APOBEC3F require an endogenous cofactor to block HIV-1 replication.; Authors - Han Yanxing Y

{{ptg:RelatedPrimaryAntibodies}}