MOV10 Rekombinanter Antikörper

MOV10 Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

250130D2

Kat-Nr. : 85818-5-RR

Synonyme

Armitage homolog, EC:3.6.4.13, fSAP113, gb110, Helicase MOV-10

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  WB analysis using 85818-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85818-5-RR (MOV10 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 85818-5-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MOV10 antibody (85818-5-RR, Clone: 250130D2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 85818-5-RR

  Biolayer interferometry (BLl) kinetic assays of 85818-5-RR against Human MOV10 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, C6-Zellen, HEK-293-Zellen, HeLa-Zellen, L02-Zellen, MCF-7-Zellen, NIH/3T3-Zellen
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

85818-5-RR bindet in WB, IF/ICC, ELISA MOV10 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: MOV10 fusion protein Ag0384
• Vollständiger Name: Mov10, Moloney leukemia virus 10, homolog (mouse)
• Berechnetes Molekulargewicht: 114 kDa
• Beobachtetes Molekulargewicht: 115 kDa
• GenBank-Zugangsnummer: BC002548
• Gene symbol: MOV10
• Gene ID (NCBI): 4343
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MOV10, also named as KIAA1631, belongs to the DNA2/NAM7 helicase family and SDE3 subfamily. It is required for RNA-mediated gene silencing by the RNA-induced silencing complex (RISC). Human MOV10 may regulate a wide range of RNA viruses and could also control the retrotransposition of endogenous retroelements in mammals (PMID:22727223). MOV10 has a broad antiretroviral activity that can target a wide range of retroviruses, and it could be actively involved in host defense against retroviral infection. MOV10 can potently inhibit HIV-1 replication at multiple stages (PMID:20215113). It is involved in the progression of telomerase-catalyzing reaction via the interaction of telomerase protein and telomere DNA.