MPG Polyklonaler Antikörper

MPG Polyklonal Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 11481-2-PBS

Synonyme

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  WB analysis of HepG2 using 11481-2-AP (same clone as 11481-2-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11481-2-AP (MPG antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11481-2-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 11481-2-AP (same clone as 11481-2-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 11481-2-AP (MPG antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11481-2-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 11481-2-AP (same clone as 11481-2-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 11481-2-AP (MPG antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11481-2-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 11481-2-AP (same clone as 11481-2-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11481-2-AP (MPG antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11481-2-PBS in a different storage buffer formulation.

• 

  IP experiment of HepG2 using 11481-2-AP (same clone as 11481-2-PBS)

  IP result of anti-MPG (IP:11481-2-AP, 4ug; Detection:11481-2-AP 1:300) with HepG2 cells lysate 2200 ug. This data was developed using the same antibody clone with 11481-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 11481-2-AP (same clone as 11481-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11481-2-AP (MPG antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11481-2-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

11481-2-PBS bindet in WB, IHC, IP, Indirect ELISA MPG und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MPG fusion protein Ag2034
• Vollständiger Name: N-methylpurine-DNA glycosylase
• Berechnetes Molekulargewicht: 293 aa, 32 kDa
• Beobachtetes Molekulargewicht: 33 kDa
• GenBank-Zugangsnummer: BC014991
• Gene symbol: MPG
• Gene ID (NCBI): 4350
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

N-methylpurine DNA glycosylase (MPG), an initiator glycosylase of the base excision repair (BER) pathway, typically repairs alkyl-induced DNA adducts, many of which are associated with genomic instability and tumorigenic risk [PMID:22801474] MPG initiates BER by removing TMZ-induced N3-methyladenine and N7-methylguanine base lesions, leaving abasic sites (AP sites) in DNA for further processing by BER [PMID:21377995]. MPG involves in modulating the genotoxic threshold response induced by the Sn2 alkylating agent ethyl methanesulfonate (EMS) [PMID:21071726].