MPP8 Polyklonaler Antikörper

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• KD/KO Validated

MPP8 Polyklonal Antikörper für WB, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen(30)

Rezensionen (3)

Kat-Nr. : 16796-1-AP

Synonyme

MPHOSPH8, Two hybrid-associated protein 3 with RanBPM, Twa3, M-phase phosphoprotein 8, HSMPP8

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• 

  WB analysis using 16796-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16796-1-AP (MPP8 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 16796-1-AP

  IP result of anti-MPP8 (IP:16796-1-AP, 4ug; Detection:16796-1-AP 1:20000) with HEK-293 cells lysate 1400 ug.

• 

  IF Staining of U2OS using 16796-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using MPP8 antibody (16796-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, MCF-7-Zellen, Maushirngewebe, NIH/3T3-Zellen, Rattenhirngewebe, U-251-Zellen
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IF/ICC: U2OS-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 6 publications below
• WB: See 22 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• IP: See 4 publications below
• CoIP: See 2 publications below
• ChIP: See 4 publications below

Produktinformation

16796-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA MPP8 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MPP8 fusion protein Ag10268
• Vollständiger Name: M-phase phosphoprotein 8
• Berechnetes Molekulargewicht: 860 aa, 97 kDa
• Beobachtetes Molekulargewicht: 97-105 kDa
• GenBank-Zugangsnummer: BC003542
• Gene symbol: MPP8
• Gene ID (NCBI): 54737
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The M-phase phosphoprotein 8 (MPP8) was firstly identified to coimmunoprecipitate with the RanBPM-comprised large protein complex [PMID:19956676]. It also binds to methylated H3K9 and recruited the H3K9 methyltransferases GLP and ESET, as well as DNA methyltransferase 3A (DNMT3A) to the promoter of the E-cadherin gene, a key regulator of tumor cell growth and epithelial-to-mesenchymal transition (EMT) [PMID:18726070]. This binding event of MPP8-H3K9me modulates the expression of E-cadherin, a key regulator of cell-cell adhesion with implications in embryonic development, tissue morphogenesis and homeostasis , together with H3K9 methyltransferases GLP and ESET and DNA methyltransferase 3a (Dnmt3a) [PMID: 18726070].

Publikationen

Species	Application	Title
human	WB	Nature; Genome surveillance by HUSH-mediated silencing of intronless mobile elements.; Authors - Marta Seczynska
human	WB,IF	Science; GENE SILENCING. Epigenetic silencing by the HUSH complex mediates position-effect variegation in human cells.; Authors - Iva A Tchasovnikarova ; KD Validated
human	WB	Nat Genet; Hyperactivation of HUSH complex function by Charcot-Marie-Tooth disease mutation in MORC2.; Authors - Iva A Tchasovnikarova
	ChIP	Nat Genet; Silencing of LINE-1 retrotransposons is a selective dependency of myeloid leukemia.; Authors - Zhimin Gu
mouse	WB	Nat Cell Biol; Distinct H3K9me3 heterochromatin maintenance dynamics govern different gene programmes and repeats in pluripotent cells; Authors - Jingchao Zhang
mouse	WB,IP	Mol Cell; Chromatin modifier HUSH co-operates with RNA decay factor NEXT to restrict transposable element expression.; Authors - William Garland

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Tsimafei (Verified Customer) (09-22-2025)

Excellent antibody!

• Applications: Western Blot
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: U2OS

MPP8 Antibody Western Blot validation (1:3000 dilution) in U2OS (Cat no:16796-1-AP)

FH

Julia (Verified Customer) (08-04-2025)

Good antibody! works great for mESC

• Applications: Western Blot
• Cell Tissue Type: mESC

MPP8 Antibody Western Blot validation ( dilution) in mESC (Cat no:16796-1-AP)

FH

Roy (Verified Customer) (06-12-2024)

Great antibody to IP MPP8, working well on WB as well (1/1000, 1h at RT) in HeLa cells and Jurkat cells.

• Applications: Western Blot, Immunoprecipitation
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: HeLa, Jurkat