MPI Polyklonaler Antikörper

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• KD/KO Validated

MPI Polyklonal Antikörper für WB, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Rezensionen (1)

Kat-Nr. : 14234-1-AP

Synonyme

Phosphomannose isomerase, Phosphohexomutase, Mannose-6-phosphate isomerase, mannose phosphate isomerase, Mannose 6 phosphate isomerase

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  WB analysis using 14234-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14234-1-AP (MPI antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 14234-1-AP

  WB result of MPI antibody (14234-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MPI transfected HEK-293 cells.

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  WB analysis of human brain using 14234-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 14234-1-AP (MPI antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 14234-1-AP

  IP result of anti-MPI (IP:14234-1-AP, 4ug; Detection:14234-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

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  IF Staining of NIH/3T3 using 14234-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed NIH/3T3 cells using 14234-1-AP (MPI antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of NIH/3T3 using 14234-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using MPI antibody (14234-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, HEK-293-Zellen, humanes Hirngewebe, Rattenhirngewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IF/ICC: NIH/3T3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below

Produktinformation

14234-1-AP bindet in WB, IF/ICC, IP, ELISA MPI und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MPI fusion protein Ag5475
• Vollständiger Name: mannose phosphate isomerase
• Berechnetes Molekulargewicht: 47 kDa
• Beobachtetes Molekulargewicht: 40-47 kDa
• GenBank-Zugangsnummer: BC046357
• Gene symbol: MPI
• Gene ID (NCBI): 4351
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MPI(mannose-6-phosphate isomerase) is also named as PMI1 and belongs to the mannose-6-phosphate isomerase type 1 family. It is a zinc metalloenzyme that catalyzes the reversible isomerization of mannose-6-phosphate and fructose-6-phosphate. MPI plays a critical role in maintaining the supply of D-mannose derivatives, which are required for most glycosylation reactions. This proterin has 2 isoforms produced by alternative splicing.

Publikationen

Species	Application	Title
human	WB	Nature; Mannose impairs tumour growth and enhances chemotherapy.; Authors - Pablo Sierra Gonzalez ; KD Validated
rat	WB	Mil Med Res; D-mannose alleviates intervertebral disc degeneration through glutamine metabolism; Authors - Zheng-Lin Dong
human	WB	Int J Biol Sci; Mannose Inhibits NSCLC Growth and Inflammatory Microenvironment by Regulating Gut Microbiota and Targeting OGT/hnRNP R/JUN/IL-8 Axis; Authors - Haoyi Jin

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Macarena Lucia (Verified Customer) (10-17-2022)

clear band

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000