MPI Monoklonaler Antikörper

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MPI Monoklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

3B10A6

Kat-Nr. : 68417-1-PBS

Synonyme

Phosphomannose isomerase, Phosphohexomutase, Mannose-6-phosphate isomerase, mannose phosphate isomerase, Mannose 6 phosphate isomerase

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  WB analysis using 68417-1-Ig (same clone as 68417-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68417-1-Ig (MPI antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68417-1-PBS in a different storage buffer formulation.

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  WB analysis of rat brain using 68417-1-Ig (same clone as 68417-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 68417-1-Ig (MPI antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68417-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 68417-1-Ig (same clone as 68417-1-PBS)

  WB result of MPI antibody (68417-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MPI transfected HEK-293 cells. This data was developed using the same antibody clone with 68417-1-PBS in a different storage buffer formulation.

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  IP experiment of Jurkat using 68417-1-Ig (same clone as 68417-1-PBS)

  IP result of anti-MPI (IP:68417-1-Ig, 4ug; Detection:68417-1-Ig 1:2000) with Jurkat cells lysate 2280 ug. This data was developed using the same antibody clone with 68417-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 68417-1-Ig (same clone as 68417-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68417-1-Ig (MPI antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68417-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 68417-1-Ig (same clone as 68417-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68417-1-Ig (MPI antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68417-1-PBS in a different storage buffer formulation.

• 

  IF Staining of NIH/3T3 using 68417-1-Ig (same clone as 68417-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed NIH/3T3 cells using MPI antibody (68417-1-Ig, Clone: 3B10A6 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68417-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

68417-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA MPI und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: MPI fusion protein Ag5475
• Vollständiger Name: mannose phosphate isomerase
• Berechnetes Molekulargewicht: 47 kDa
• Beobachtetes Molekulargewicht: 40-47 kDa
• GenBank-Zugangsnummer: BC046357
• Gene symbol: MPI
• Gene ID (NCBI): 4351
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MPI(mannose-6-phosphate isomerase) is also named as PMI1 and belongs to the mannose-6-phosphate isomerase type 1 family. It is a zinc metalloenzyme that catalyzes the reversible isomerization of mannose-6-phosphate and fructose-6-phosphate. MPI plays a critical role in maintaining the supply of D-mannose derivatives, which are required for most glycosylation reactions. This proterin has 2 isoforms produced by alternative splicing.