MPO Polyklonaler Antikörper

MPO Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (3)

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

90

Kat-Nr. : 22225-1-AP

Synonyme

MPO, myeloperoxidase

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Galerie der Validierungsdaten

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  WB analysis of HL-60 using 22225-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 22225-1-AP (MPO antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U-937 using 22225-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 22225-1-AP (MPO antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HL-60 using 22225-1-AP

  IP result of anti-MPO(IP:22225-1-AP, 4ug; Detection:22225-1-AP 1:4000) with HL-60 cells lysate 1720 ug.

• 

  IHC staining of human colon cancer using 22225-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22225-1-AP (MPO antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 22225-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22225-1-AP (MPO antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 22225-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 22225-1-AP (MPO antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 22225-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 22225-1-AP (MPO antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human spleen using 22225-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 22225-1-AP (MPO antibody) at dilution of 1:800 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human spleen using 22225-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 22225-1-AP (MPO antibody) at dilution of 1:800 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 22225-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 22225-1-AP (MPO antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 22225-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 22225-1-AP (MPO antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of human tonsillitis using 22225-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 22225-1-AP (MPO antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human tonsillitis using 22225-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 22225-1-AP (MPO antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HL-60-Zellen, U-937-Zellen
• Erfolgreiche IP: HL-60-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, humanes Lebergewebe, humanes Milzgewebe, Maus-Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: humanes Tonsillitisgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 21 publications below
• IHC: See 47 publications below
• IF: See 30 publications below
• ELISA: See 3 publications below
• FC: See 3 publications below

Produktinformation

22225-1-AP bindet in WB, IP, IHC, IF, FC, ELISA MPO und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, hamster, Ratte, Rind
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MPO fusion protein Ag17564
• Vollständiger Name: myeloperoxidase
• Berechnetes Molekulargewicht: 745 aa, 84 kDa
• Beobachtetes Molekulargewicht: 59 kDa
• GenBank-Zugangsnummer: BC130476
• Gene symbol: MPO
• Gene ID (NCBI): 4353
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The MPO gene encodes myeloperoxidase, a lysosomal hemoprotein located in the azurophilic granules of polymorphonuclear (PMN) leukocytes and monocytes. In response to stimulation, MPO is activated into a transient intermediate with potent antimicrobial oxidizing abilities(PMID:17650507). The mRNA is translated into a single protein of 90 kDa, which displays enzymatic activity and undergoes proteolytic maturation into a heavy chain of 59 kDa and a light chain of 13.5 kDa; these subunits then dimerize into the mature tetramer and the mature MPO is a heterotetramer composed of two identical heavy chains and two identical light chains(PMID:12773517). Fragments with molecular masses of 43-47 kDa were formed by autocatalysis during warming in sample buffer (PMID:12960244). The 24-kDa material had a map identical to that of 13.5 kDa subunit and represents a dimer of the 13.5 kDa subunit (PMID:3008892). Defects in MPO are the cause of myeloperoxidase deficiency (MPOD). It has 3 isoforms produced by alternative splicing. This antibody is specific to MPO.

Publikationen

Species	Application	Title
	WB	Crit Care; Recombinant ACE2 protein protects against acute lung injury induced by SARS-CoV-2 spike RBD protein.; Authors - Lingbing Zhang
human	IHC	Blood; C1Q labels a highly aggressive macrophage-like leukemia population indicating extramedullary infiltration and relapse; Authors - Li-Xue Yang
	WB,IHC	Acta Pharm Sin B; A new perspective of triptolide-associated hepatotoxicity: the relevance of NF- κ B and NF- κ B-mediated cellular FLICE-inhibitory protein.; Authors - Ziqiao Yuan
	IHC,IF	Acta Pharm Sin B; A non-human primate derived anti-P-selectin glycoprotein ligand-1 antibody curtails acute pancreatitis by alleviating the inflammatory responses; Authors - Yuhan Li
	IF	Sci Adv; Astrocytic NDRG2-PPM1A interaction exacerbates blood-brain barrier disruption after subarachnoid hemorrhage; Authors - Dayun Feng
rat	IHC	Acta Biomater; An electrospun scaffold functionalized with a ROS-scavenging hydrogel stimulates ocular wound healing; Authors - Xin Shi