MPO Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

MPO Monoklonal Antikörper für WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA

Wirt / Isotyp

Maus / IgA

Getestete Reaktivität

human, Ratte und mehr (2)

Anwendung

WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA

Konjugation

Unkonjugiert

Publikationen(59)

CloneNo.

4C11F6

Kat-Nr. : 66177-1-Ig

Synonyme

4C11F6, EC:1.11.2.2, myeloperoxidase

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  WB analysis of HL-60 using 66177-1-Ig

  HL-60 cells were subjected to SDS PAGE followed by western blot with 66177-1-Ig (MPO antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 66177-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66177-1-Ig (MPO antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 66177-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66177-1-Ig (MPO antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 66177-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66177-1-Ig (MPO antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 66177-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66177-1-Ig (MPO antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 66177-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66177-1-Ig (MPO antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human appendicitis using 66177-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human appendicitis tissue using MPO antibody (66177-1-Ig, Clone: 4C11F6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HL-60 using 66177-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HL-60 cells using MPO antibody (66177-1-Ig, Clone: 4C11F6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

• 

  FC experiment of HL-60 using 66177-1-Ig

  1x10^6 HL-60 cells were intracellularly stained with 0.25 ug MPO Monoclonal antibody (66177-1-Ig, Clone:4C11F6) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HL-60-Zellen
• Erfolgreiche Detektion in IHC: humanes Lebergewebe, humanes Tonsillitisgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: humanes Appendizitis-Gewebe
• Erfolgreiche Detektion in IF/ICC: HL-60-Zellen
• Erfolgreiche Detektion in FC (Intra): HL-60-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunhistochemie (IHC): IHC : 1:400-1:1600
• Immunfluoreszenz (IF)-P: IF-P : 1:200-1:800
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 13 publications below
• IHC: See 18 publications below
• IF: See 40 publications below
• IP: See 1 publications below
• ELISA: See 1 publications below

Produktinformation

66177-1-Ig bindet in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA MPO und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• In Publikationen genannte Reaktivität: human, Ratte, Rind, Kuh
• Wirt / Isotyp: Maus / IgA
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: MPO fusion protein Ag17564
• Vollständiger Name: myeloperoxidase
• Berechnetes Molekulargewicht: 745 aa, 84 kDa
• Beobachtetes Molekulargewicht: 90 kDa
• GenBank-Zugangsnummer: BC130476
• Gene symbol: MPO
• Gene ID (NCBI): 4353
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Caprylsäure/Ammoniumsulfat-Präzipitation
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The MPO gene encodes myeloperoxidase, a lysosomal hemoprotein located in the azurophilic granules of polymorphonuclear (PMN) leukocytes and monocytes. In response to stimulation, MPO is activated into a transient intermediate with potent antimicrobial oxidizing abilities(PMID:17650507). The mRNA is translated into a single protein of 90 kDa, which displays enzymatic activity and undergoes proteolytic maturation into a heavy chain of 59 kDa and a light chain of 13.5 kDa; these subunits then dimerize into the mature tetramer and the mature MPO is a heterotetramer composed of two identical heavy chains and two identical light chains(PMID:12773517). Fragments with molecular masses of 43-47 kDa were formed by autocatalysis during warming in sample buffer (PMID:12960244). The 24-kDa material had a map identical to that of 13.5 kDa subunit and represents a dimer of the 13.5 kDa subunit (PMID:3008892). Defects in MPO are the cause of myeloperoxidase deficiency (MPOD). It has 3 isoforms produced by alternative splicing.

Publikationen

Species	Application	Title
	IF	Nat Commun; Overcoming therapeutic resistance in oncolytic herpes virotherapy by targeting IGF2BP3-induced NETosis in malignant glioma; Authors - Weiwei Dai
rat	IF	Cell Death Differ; NLRX1 mediated impaired microglial phagocytosis of NETs in cerebral ischemia and reperfusion injury; Authors - Jialing Peng
		PLoS Pathog; Prion protein signaling induces M2 macrophage polarization and protects from lethal influenza infection in mice.; Authors - Junji Chida
	IHC	Int Immunopharmacol; Sappanone A enhances hepatocyte proliferation in lipopolysaccharide-induced acute liver injury in mice by promoting injured hepatocyte apoptosis and regulating macrophage polarization; Authors - Jiale He
	IF	FEBS J; A combined model of serum neutrophil extracellular traps, CD8+ T cells, and tumor proportion score provides better prediction of PD-1 inhibitor efficacy in patients with NSCLC; Authors - Jian Guo
human	IHC	Sci Rep; Persisting and Increasing Neutrophil Infiltration Associates with Gastric Carcinogenesis and E-cadherin Downregulation.; Authors - Hualin Fu