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MPO Rekombinanter Antikörper
MPO Rekombinant Antikörper für WB, IF/ICC, FC (Intra), ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human und mehr (1)
Anwendung
WB, IF/ICC, FC (Intra), ELISA
Konjugation
Unkonjugiert
CloneNo.
3J17
Kat-Nr. : 81610-1-RR
Synonyme
Geprüfte Anwendungen
Erfolgreiche Detektion in WB | HL-60-Zellen, human saliva |
Erfolgreiche Detektion in IF/ICC | HL-60-Zellen |
Erfolgreiche Detektion in FC (Intra) | HL-60-Zellen |
Empfohlene Verdünnung
Anwendung | Verdünnung |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunfluoreszenz (IF)/ICC | IF/ICC : 1:800-1:3200 |
Durchflusszytometrie (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Veröffentlichte Anwendungen
WB | See 1 publications below |
IF | See 2 publications below |
Produktinformation
81610-1-RR bindet in WB, IF/ICC, FC (Intra), ELISA MPO und zeigt Reaktivität mit human
Getestete Reaktivität | human |
In Publikationen genannte Reaktivität | human, Maus |
Wirt / Isotyp | Kaninchen / IgG |
Klonalität | Rekombinant |
Typ | Antikörper |
Immunogen | MPO fusion protein Ag17564 |
Vollständiger Name | myeloperoxidase |
Berechnetes Molekulargewicht | 745 aa, 84 kDa |
Beobachtetes Molekulargewicht | 59 kDa, 90 kDa |
GenBank-Zugangsnummer | BC130476 |
Gene symbol | MPO |
Gene ID (NCBI) | 4353 |
Konjugation | Unkonjugiert |
Form | Liquid |
Reinigungsmethode | Protein-A-Reinigung |
Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol |
Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
The MPO gene encodes myeloperoxidase, a lysosomal hemoprotein located in the azurophilic granules of polymorphonuclear (PMN) leukocytes and monocytes. In response to stimulation, MPO is activated into a transient intermediate with potent antimicrobial oxidizing abilities(PMID:17650507). The mRNA is translated into a single protein of 90 kDa, which displays enzymatic activity and undergoes proteolytic maturation into a heavy chain of 59 kDa and a light chain of 13.5 kDa; these subunits then dimerize into the mature tetramer and the mature MPO is a heterotetramer composed of two identical heavy chains and two identical light chains(PMID:12773517). Fragments with molecular masses of 43-47 kDa were formed by autocatalysis during warming in sample buffer (PMID:12960244).The 24-kDa material had a map identical to that of 13.5 kDa subunit and represents a dimer of the 13.5 kDa subunit (PMID:3008892). Defects in MPO are the cause of myeloperoxidase deficiency (MPOD). It has 3 isoforms produced by alternative splicing.
Protokolle
PRODUKTSPEZIFISCHE PROTOKOLLE | |
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WB protocol for MPO antibody 81610-1-RR | Protokoll herunterladen |
IF protocol for MPO antibody 81610-1-RR | Protokoll herunterladen |
STANDARD-PROTOKOLLE | |
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Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
Species | Application | Title |
---|---|---|
Placenta NETs exacerbate placental inflammation and injury through high mobility group protein B1 during preeclampsia | ||
Free Radic Biol Med Neutrophil extracellular traps induce fibroblast ferroptosis via IRE1α/XBP1-mediated ER stress to impair diabetic wound healing |