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MPO Rekombinanter Antikörper

MPO Rekombinant Antikörper für WB, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

Publikationen(2)

CloneNo.

3J17

Kat-Nr. : 81610-1-RR

Synonyme

3J17, EC:1.11.2.2, myeloperoxidase



Geprüfte Anwendungen

Erfolgreiche Detektion in WBHL-60-Zellen, human saliva
Erfolgreiche Detektion in IF/ICCHL-60-Zellen
Erfolgreiche Detektion in FC (Intra)HL-60-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:5000-1:50000
Immunfluoreszenz (IF)/ICCIF/ICC : 1:800-1:3200
Durchflusszytometrie (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

WBSee 1 publications below
IFSee 2 publications below

Produktinformation

81610-1-RR bindet in WB, IF/ICC, FC (Intra), ELISA MPO und zeigt Reaktivität mit human

Getestete Reaktivität human
In Publikationen genannte Reaktivitäthuman, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen MPO fusion protein Ag17564
Vollständiger Name myeloperoxidase
Berechnetes Molekulargewicht 745 aa, 84 kDa
Beobachtetes Molekulargewicht 59 kDa, 90 kDa
GenBank-ZugangsnummerBC130476
Gene symbol MPO
Gene ID (NCBI) 4353
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The MPO gene encodes myeloperoxidase, a lysosomal hemoprotein located in the azurophilic granules of polymorphonuclear (PMN) leukocytes and monocytes. In response to stimulation, MPO is activated into a transient intermediate with potent antimicrobial oxidizing abilities(PMID:17650507). The mRNA is translated into a single protein of 90 kDa, which displays enzymatic activity and undergoes proteolytic maturation into a heavy chain of 59 kDa and a light chain of 13.5 kDa; these subunits then dimerize into the mature tetramer and the mature MPO is a heterotetramer composed of two identical heavy chains and two identical light chains(PMID:12773517). Fragments with molecular masses of 43-47 kDa were formed by autocatalysis during warming in sample buffer (PMID:12960244).The 24-kDa material had a map identical to that of 13.5 kDa subunit and represents a dimer of the 13.5 kDa subunit (PMID:3008892). Defects in MPO are the cause of myeloperoxidase deficiency (MPOD). It has 3 isoforms produced by alternative splicing.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for MPO antibody 81610-1-RRProtokoll herunterladen
IF protocol for MPO antibody 81610-1-RRProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanWB,IF

Placenta

NETs exacerbate placental inflammation and injury through high mobility group protein B1 during preeclampsia

Authors - Yurong Lu
mouseIF

Free Radic Biol Med

Neutrophil extracellular traps induce fibroblast ferroptosis via IRE1α/XBP1-mediated ER stress to impair diabetic wound healing

Authors - Hongbo Zhao