MPO Rekombinanter Antikörper

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• KD/KO Validated

MPO Rekombinant Antikörper für WB, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

Publikationen(2)

CloneNo.

3J17

Kat-Nr. : 81610-1-RR

Synonyme

3J17, EC:1.11.2.2, myeloperoxidase

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  WB analysis of HL-60 using 81610-1-RR

  HL-60 cells were subjected to SDS PAGE followed by western blot with 81610-1-RR (MPO antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human saliva using 81610-1-RR

  human saliva were subjected to SDS PAGE followed by western blot with 81610-1-RR (MPO antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 81610-1-RR

  WB result of MPO antibody (81610-1-RR; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MPO transfected HL-60 cells.

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  IF Staining of HL-60 using 81610-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HL-60 cells using MPO antibody (81610-1-RR, Clone: 3J17 ) at dilution of 1:1600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HL-60 using 81610-1-RR

  1x10^6 HL-60 cells were intracellularly stained with 0.25 ug MPO Recombinant antibody (81610-1-RR, Clone:3J17) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  Affinity and Kinetic Characterization of 81610-1-RR

  Biolayer interferometry (BLl) kinetic assays of 81610-1-RR against Human MPO were performed. The affinity constant is 3.87 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HL-60-Zellen, human saliva
• Erfolgreiche Detektion in IF/ICC: HL-60-Zellen
• Erfolgreiche Detektion in FC (Intra): HL-60-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:800-1:3200
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IF: See 2 publications below

Produktinformation

81610-1-RR bindet in WB, IF/ICC, FC (Intra), ELISA MPO und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: MPO fusion protein Ag17564
• Vollständiger Name: myeloperoxidase
• Berechnetes Molekulargewicht: 745 aa, 84 kDa
• Beobachtetes Molekulargewicht: 59 kDa, 90 kDa
• GenBank-Zugangsnummer: BC130476
• Gene symbol: MPO
• Gene ID (NCBI): 4353
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The MPO gene encodes myeloperoxidase, a lysosomal hemoprotein located in the azurophilic granules of polymorphonuclear (PMN) leukocytes and monocytes. In response to stimulation, MPO is activated into a transient intermediate with potent antimicrobial oxidizing abilities(PMID:17650507). The mRNA is translated into a single protein of 90 kDa, which displays enzymatic activity and undergoes proteolytic maturation into a heavy chain of 59 kDa and a light chain of 13.5 kDa; these subunits then dimerize into the mature tetramer and the mature MPO is a heterotetramer composed of two identical heavy chains and two identical light chains(PMID:12773517). Fragments with molecular masses of 43-47 kDa were formed by autocatalysis during warming in sample buffer (PMID:12960244).The 24-kDa material had a map identical to that of 13.5 kDa subunit and represents a dimer of the 13.5 kDa subunit (PMID:3008892). Defects in MPO are the cause of myeloperoxidase deficiency (MPOD). It has 3 isoforms produced by alternative splicing.

Publikationen

Species	Application	Title
human	WB,IF	Placenta; NETs exacerbate placental inflammation and injury through high mobility group protein B1 during preeclampsia; Authors - Yurong Lu
mouse	IF	Free Radic Biol Med; Neutrophil extracellular traps induce fibroblast ferroptosis via IRE1α/XBP1-mediated ER stress to impair diabetic wound healing; Authors - Hongbo Zhao