MPO Rekombinanter Antikörper

MPO Rekombinant Antikörper für Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

240229A6

Kat-Nr. : 83282-5-PBS

Synonyme

EC:1.11.2.2, myeloperoxidase

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  Sandwich ELISA standard curve of MP00321-2

  Sandwich ELISA standard curve of MP00321-2, Human MPO Recombinant Matched Antibody Pair - PBS only. 83282-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0511. 83282-5-PBS was HRP conjugated as the detection antibody. Range: 39-2500 pg/mL.

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  Sample test of MP00321-2

  Human peripheral blood mononuclear cells (PBMC) were cultured unstimulated or stimulated with 10 μg/mL PHA for 3 days or 5 days. For the PBMC supernatant cultured for 3 days, the mean MPO concentration was determined to be 6.6 ng/mL in unstimulated PBMC supernatant, 20.0 ng/mL in PHA stimulated PBMC supernatant. For the PBMC supernatant cultured for 5 days, the mean MPO concentration was determined to be 7.2 ng/mL in unstimulated PBMC supernatant, 28.2 ng/mL in PHA stimulated PBMC supernatant.

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  Sample test of MP00321-2

  Urine of six individual healthy human donors was measured. The MPO concentration of detected samples was determined to be 2,249.9 pg/mL with a range of 294.1 - 6,991.1 pg/mL.

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  Sample test of MP00321-2

  Saliva of eight individual healthy human donors was measured. The MPO concentration of detected samples was determined to be 314.7 ng/mL with a range of 6.3 - 1,446.1 ng/mL.

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  Sample test of MP00321-2

  The mean MPO concentration was determined to be 73,583.3 ng/mL in HL-60 cell extract based on a 4.3 mg/mL extract load.

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  Sample test of MP00321-2

  Serum of sixteen individual healthy human donors was measured. The MPO concentration of detected samples was determined to be 169.9 ng/mL with a range of 54.5 - 419.2 ng/mL.

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  Affinity and Kinetic Characterization of 83282-5-PBS

  Biolayer interferometry (BLl) kinetic assay of 83282-5-PBS against Human MPO was performed. The affinity constant is 1.27 nM.

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Geprüfte Anwendungen

Produktinformation

83282-5-PBS bindet in Sandwich ELISA, Indirect ELISA, Sample test MPO und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: MPO fusion protein Eg0511
• Vollständiger Name: myeloperoxidase
• Berechnetes Molekulargewicht: 745 aa, 84 kDa
• GenBank-Zugangsnummer: BC130476
• Gene symbol: MPO
• Gene ID (NCBI): 4353
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.