MPO Rekombinanter Antikörper

MPO Rekombinant Antikörper für WB, IHC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, ELISA

Konjugation

Unkonjugiert

CloneNo.

241839C11

Kat-Nr. : 84436-5-RR

Synonyme

Myeloperoxidase light chain, Myeloperoxidase heavy chain, Myeloperoxidase, EC:1.11.2.2, 241839C11

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  WB analysis using 84436-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84436-5-RR (MPO antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse spleen using 84436-5-RR

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84436-5-RR (Mpo antibody) at dilution of 1:250 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse spleen using 84436-5-RR

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84436-5-RR (Mpo antibody) at dilution of 1:250 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse spleen using 84436-5-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using Mpo antibody (84436-5-RR, Clone: 241839C11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 84436-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84436-5-RR against Mouse Mpo were performed. The affinity constant is 23.5 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HL-60-Zellen, Mausmilzgewebe, Rattenmilzgewebe
• Erfolgreiche Detektion in IHC: Mausmilzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Mausmilzgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:125-1:500
• Immunfluoreszenz (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84436-5-RR bindet in WB, IHC, IF-P, ELISA MPO und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: myeloperoxidase
• Berechnetes Molekulargewicht: 81KD
• Beobachtetes Molekulargewicht: 43 kDa, 59 kDa, 90 kDa
• GenBank-Zugangsnummer: AAR99349
• Gene symbol: Mpo
• Gene ID (NCBI): 17523
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The MPO gene encodes myeloperoxidase, a lysosomal hemoprotein located in the azurophilic granules of polymorphonuclear (PMN) leukocytes and monocytes. In response to stimulation, MPO is activated into a transient intermediate with potent antimicrobial oxidizing abilities(PMID:17650507). The mRNA is translated into a single protein of 90 kDa, which displays enzymatic activity and undergoes proteolytic maturation into a heavy chain of 59 kDa and a light chain of 13.5 kDa; these subunits then dimerize into the mature tetramer and the mature MPO is a heterotetramer composed of two identical heavy chains and two identical light chains(PMID:12773517). Fragments with molecular masses of 43-47 kDa were formed by autocatalysis during warming in sample buffer (PMID:12960244). The 24-kDa material had a map identical to that of 13.5 kDa subunit and represents a dimer of the 13.5 kDa subunit (PMID:3008892). Defects in MPO are the cause of myeloperoxidase deficiency (MPOD). It has 3 isoforms produced by alternative splicing.