MPRIP Rekombinanter Antikörper

MPRIP Rekombinant Antikörper für WB, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF-P, ELISA

Konjugation

Unkonjugiert

CloneNo.

250643B2

Kat-Nr. : 86033-1-RR

Synonyme

M RIP, MRIP, M-RIP, Myosin phosphatase Rho-interacting protein, p116Rip

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  WB analysis using 86033-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86033-1-RR (MPRIP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IF Staining of mouse brain using 86033-1-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using MPRIP antibody (86033-1-RR, Clone: 250643B2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, A549-Zellen, SKOV-3-Zellen
• Erfolgreiche Detektion in IF-P: Maushirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunfluoreszenz (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

86033-1-RR bindet in WB, IF-P, ELISA MPRIP und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: myosin phosphatase Rho interacting protein
• Berechnetes Molekulargewicht: 117 kDa
• Beobachtetes Molekulargewicht: 140 kDa
• GenBank-Zugangsnummer: NM_015134
• Gene symbol: MPRIP
• Gene ID (NCBI): 23164
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MPRIP, also named as KIAA0864, MRIP, RHOIP3 and p116Rip, is required for the regulation of the actin cytoskeleton by RhoA and ROCK1. MRPIP targets myosin phosphatase to the actin cytoskeleton. Depletion leads to an increased number of stress fibers in smooth muscle cells through stabilization of actin fibers by phosphorylated myosin. Overexpression of MRIP as well as its F-actin-binding region leads to disassembly of stress fibers in neuronal cells. The antibody recognizes the C-term of isoform2 and isoform3 of MPRIP.