MPZ / P0 Polyklonaler Antikörper

MPZ / P0 Polyklonal Antikörper für IF, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

14

Kat-Nr. : 10572-1-AP

Synonyme

CHM, CMT1, CMT1B, CMT2I, CMT2J, CMT4E, CMTDI3, DSS, HMSNIB, MPP, MPZ, MPZ / P0, Myelin peripheral protein, Myelin protein P0, myelin protein zero, P0

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Galerie der Validierungsdaten

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  WB analysis of Schwann cells using 10572-1-AP

  WB result from Chattopadhyay S (PMID:19229995); Schwann cell differentiation was induced with dbcAMP (500 μM) for 48 h, followed by treatment with rhMMP-9 (100 nM) for 15 min. A, rhMMP-9 stimulates transient ERK1/2 activation over a 1 h period. Successful Schwann cell differentiation was confirmed by myelin protein zero (P0) expression, β-actin was used as loading control.

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  IF Staining of myelinating SCs cells using 10572-1-AP

  IF result from Huaqing Liu (PMID:20448483); Dual immunofluorescence for BrdU (red) with myelinating Schwann cell markers P0 (10572-1-AP, green, 1:150) in distal segments of MMPi-treated nerves. .

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Schwannzellen
• Erfolgreiche Detektion in IF: Myelinisierende SCs-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 10 publications below
• IHC: See 1 publications below
• IF: See 5 publications below

Produktinformation

10572-1-AP bindet in WB, IHC, IF, ELISA MPZ / P0 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte, Frosch
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MPZ / P0 fusion protein Ag0848
• Vollständiger Name: myelin protein zero
• Berechnetes Molekulargewicht: 28 kDa
• Beobachtetes Molekulargewicht: 30 kDa
• GenBank-Zugangsnummer: BC006491
• Gene symbol: MPZ
• Gene ID (NCBI): 4359
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MPZ (myelin protein zero), also known as P0, a transmembrane glycoprotein (~30 kDa), is a member of the immunoglobulin supergene family. Synthesized by myelin-forming Schwann cells, MPZ is the major structural protein component of myelin in the peripheral nervous system. It is involved in formation and maintenance of compact myelin, and plays a role in the creation of an extracellular membrane face which guides the wrapping process and ultimately compacts adjacent lamellae. More than 120 mutations detected in the gene of MPZ cause various forms of hereditary neuropathy, which include Charcot-Marie-Tooth disease type 1B (CMT1B), CMT2, Dejerine-Sottas syndrome (DSS), and congenital hypomyelination neuropathy (CHN). This antibody can recognize endogenous MPZ, and can be used as a marker of myelinating Schwann cells.

Publikationen

Species	Application	Title
rat	WB	Oxid Med Cell Longev; Neuroprotective Effect of Salvianolic Acid A against Diabetic Peripheral Neuropathy through Modulation of Nrf2.; Authors - Chunyang Xu
rat	WB	Front Cell Neurosci; Protein kinase C epsilon activation regulates proliferation, migration, and epithelial to mesenchymal-like transition in rat Schwann cells; Authors - Tasnim Mohamed
human	WB	Front Endocrinol (Lausanne); Clinical identification of expressed proteins in adrenal medullary hyperplasia detected with hypertension; Authors - He Ma
rat	IF	Stem Cell Res Ther; Schwann cells promote prevascularization and osteogenesis of tissue-engineered bone via bone marrow mesenchymal stem cell-derived endothelial cells.; Authors - Xinxin Zhang
rat	WB	Glia; MMP-9 controls Schwann cell proliferation and phenotypic remodeling via IGF-1 and ErbB receptor-mediated activation of MEK/ERK pathway.; Authors - Chattopadhyay Sharmila S
mouse	WB,IF	Mol Cell Biol; DHTKD1 deficiency causes Charcot-Marie-Tooth disease in mice.; Authors - Wang-Yang Xu