MPZ / P0 Polyklonaler Antikörper

MPZ / P0 Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 10572-1-PBS

Synonyme

MPZ, Myelin protein zero, CHM, MPP, Myelin peripheral protein

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  WB analysis using 10572-1-AP (same clone as 10572-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10572-1-AP (MPZ / P0 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10572-1-PBS in a different storage buffer formulation.

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  IHC staining of rat Ischiadic nerve using 10572-1-AP (same clone as 10572-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat Ischiadic nerve tissue slide using 10572-1-AP (MPZ / P0 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10572-1-PBS in a different storage buffer formulation.

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  IHC staining of rat Ischiadic nerve using 10572-1-AP (same clone as 10572-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat Ischiadic nerve tissue slide using 10572-1-AP (MPZ / P0 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10572-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse Ischiadic nerve using 10572-1-AP (same clone as 10572-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse Ischiadic nerve tissue slide using 10572-1-AP (MPZ / P0 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10572-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse Ischiadic nerve using 10572-1-AP (same clone as 10572-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse Ischiadic nerve tissue slide using 10572-1-AP (MPZ / P0 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10572-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

10572-1-PBS bindet in WB, IHC, Indirect ELISA MPZ / P0 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MPZ / P0 fusion protein Ag0848
• Vollständiger Name: myelin protein zero
• Berechnetes Molekulargewicht: 28 kDa
• Beobachtetes Molekulargewicht: 28-30 kDa
• GenBank-Zugangsnummer: BC006491
• Gene symbol: MPZ
• Gene ID (NCBI): 4359
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MPZ (myelin protein zero), also known as P0, is a transmembrane glycoprotein that belongs to the immunoglobulin supergene family. Synthesized by myelin-forming Schwann cells, MPZ is the major structural protein component of myelin in the peripheral nervous system. It is involved in the formation and maintenance of compact myelin and plays a role in the creation of an extracellular membrane face which guides the wrapping process and ultimately compacts adjacent lamellae. More than 120 mutations detected in the gene of MPZ cause various forms of hereditary neuropathy, which include Charcot-Marie-Tooth disease type 1B (CMT1B), CMT2, Dejerine-Sottas syndrome (DSS), and congenital hypomyelination neuropathy (CHN). This antibody can recognize endogenous MPZ and can be used as a marker of myelinating Schwann cells. MPZ can be detected the 25-30 kDa band by western blot. A band of 36 kDa could also be detected, which is a novel isoform of MPZ that contains an additional domain at the C-terminal (PMID: 22457349). It can also exist as a dimer (PMID: 12933931).