MRC2 Rekombinanter Antikörper

MRC2 Rekombinant Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

250494D5

Kat-Nr. : 86028-1-RR

Synonyme

C type mannose receptor 2, CD280, CLEC13E, C-type lectin domain family 13 member E, C-type mannose receptor 2

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  WB analysis using 86028-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86028-1-RR (MRC2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 86028-1-RR

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 86028-1-RR (MRC2 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 86028-1-RR

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 86028-1-RR (MRC2 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Saos-2 cells, Mauslungengewebe, Rattenlungengewebe, U2OS-Zellen
• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

86028-1-RR bindet in WB, IHC, ELISA MRC2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: MRC2 fusion protein Eg2653
• Vollständiger Name: mannose receptor, C type 2
• Berechnetes Molekulargewicht: 167 kDa
• Beobachtetes Molekulargewicht: 180 kDa
• GenBank-Zugangsnummer: NM_006039.5
• Gene symbol: MRC2
• Gene ID (NCBI): 9902
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

C-type mannose receptor 2 (MRC2), also known as CD280, CLEC13E, ENDO180, KIAA0709, or UPARAP, is a type I transmembrane glycoprotein that belongs to the macrophage mannose receptor protein family (PMID: 10683150; 10636902; 9734811). It contains an N-terminal cysteine-rich domain, a fibronectin type II domain (FNII), eight C-type-lectin-like domains, and a transmembrane region followed by a small cytoplasmic domain (PMID: 34012764). MRC2 is a recycling endocytic receptor that functions in both these cellular activities by promoting cell migration and uptake of collagens for intracellular degradation (PMID: 17974964). MRC2 is expressed in several cells and organs including fibroblasts, endothelial cells, and macrophages. Altered MRC2 expression has been associated with the development and progression of different cancers, including head and neck squamous cell carcinoma, breast cancer, hepatocellular cancer, and prostate cancer (PMID: 36188226; 37666484; 17974964; 25162823).