MRE11 Polyklonaler Antikörper

MRE11 Polyklonal Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 10744-1-PBS

Synonyme

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  WB analysis using 10744-1-AP (same clone as 10744-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10744-1-AP (MRE11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10744-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 10744-1-AP (same clone as 10744-1-PBS)

  IP result of anti-MRE11 (IP:10744-1-AP, 4ug; Detection:10744-1-AP 1:600) with HeLa cells lysate 1200ug. This data was developed using the same antibody clone with 10744-1-PBS in a different storage buffer formulation.

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  IHC staining of human lymphoma using 10744-1-AP (same clone as 10744-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 10744-1-AP (MRE11 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 10744-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 10744-1-AP (same clone as 10744-1-PBS)

  Immunofluorescent analysis of A431 cells using 10744-1-AP (MRE11 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 10744-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

10744-1-PBS bindet in WB, IHC, IP, Indirect ELISA MRE11 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MRE11 fusion protein Ag1105
• Vollständiger Name: MRE11 meiotic recombination 11 homolog A (S. cerevisiae)
• Berechnetes Molekulargewicht: 81 kDa
• Beobachtetes Molekulargewicht: 81 kDa
• GenBank-Zugangsnummer: BC005241
• Gene symbol: MRE11A
• Gene ID (NCBI): 4361
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Mre11 (meiotic recombination 11), also named as Mre11A, is a component of the MRN( MRE11/RAD50/NBS1) complex which is a versatile complex, playing a key role in the sensing, processing, and repair of DSBs. MRE11 possesses both endonuclease and 3'-5' exonuclease activity. It contains two DNA-binding domains (DBDs), enabling it to bind both single-stranded DNA (ssDNA) and double-stranded DNA (dsDNA). After DSBs, MRE11 is loaded onto DSBs sites and cleaves DNA by cooperating with CtIP (CtBP [C terminal binding protein] interacting protein) to initiate end resection. It's reported that MRE11 is over-expressed in breast cancers. (PMID:38128537; 22914783)