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MRI1 Polyklonaler Antikörper

MRI1 Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 19916-1-PBS

Synonyme

Filter:
  • Western Blot (WB) analysis of HT-1080 cells using MRI1 Polyclonal antibody (19916-1-AP)

    WB analysis of HT-1080 using 19916-1-AP (same clone as 19916-1-PBS)

    HT-1080 cells were subjected to SDS PAGE followed by western blot with 19916-1-AP (MRI1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19916-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HL-60 cells using MRI1 Polyclonal antibody (19916-1-AP)

    WB analysis of HL-60 using 19916-1-AP (same clone as 19916-1-PBS)

    HL-60 cells were subjected to SDS PAGE followed by western blot with 19916-1-AP (MRI1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19916-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat kidney tissue using MRI1 Polyclonal antibody (19916-1-AP)

    IHC staining of rat kidney using 19916-1-AP (same clone as 19916-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 19916-1-AP (MRI1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19916-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat kidney tissue using MRI1 Polyclonal antibody (19916-1-AP)

    IHC staining of rat kidney using 19916-1-AP (same clone as 19916-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 19916-1-AP (MRI1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19916-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

19916-1-PBS bindet in WB, IHC, Indirect ELISA MRI1 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen MRI1 fusion protein Ag13809
Vollständiger Name methylthioribose-1-phosphate isomerase homolog (S. cerevisiae)
Berechnetes Molekulargewicht 369 aa, 39 kDa
Beobachtetes Molekulargewicht 45 kDa
GenBank-ZugangsnummerBC001703
Gene symbol MRI1
Gene ID (NCBI) 84245
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MRI1 is also named as MRDI and belongs to the eIF-2B alpha/beta/delta subunits family. MRI1 promotes cell invasion in response to constitutive RhoA activation by promoting FAK tyrosine phosphorylation (PMID:19620624). MRDI acts as a methylthioribose-1-phosphate (MTR-1-P) isomerase, catalyzing a critical step in methionine salvage. Second, MRDI promotes and is necessary for melanoma cell invasion, independent of its catalytic activity (PMID:23859498).