MRP1 Polyklonaler Antikörper

MRP1 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 27825-1-AP

Synonyme

ABC29, ABCC, ABCC1, GS X, Leukotriene C(4) transporter, LTC4 transporter, MRP, MRP1

---

• 

  WB analysis using 27825-1-AP

  37°C incubated HeLa cells were subjected to SDS PAGE followed by western blot with 27825-1-AP (MRP1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 27825-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27825-1-AP (ABCC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 27825-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27825-1-AP (ABCC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: 37°C incubated HeLa cells
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

27825-1-AP bindet in WB, IHC, ELISA MRP1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MRP1 fusion protein Ag27048
• Vollständiger Name: ATP-binding cassette, sub-family C (CFTR/MRP), member 1
• Berechnetes Molekulargewicht: 172 kDa
• Beobachtetes Molekulargewicht: 180-190 kDa
• GenBank-Zugangsnummer: NM_004996
• Gene symbol: MRP1
• Gene ID (NCBI): 4363
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Multidrug resistant-associated protein 1 (MRP1, also known as ABCC1) is a member of the ATP-binding cassette (ABC) transporter protein superfamily, subfamily C. MRP1 is overexpressed in cancers and contributes to the occurrence of multidrug resistance (MDR). Expression of MRP1 has been considered as a negative marker for the outcome of chemotherapy. Recently MRP1 has been reported to play a crucial role in Aβ clearance at the blood-brain barrier.

Publikationen

Species	Application	Title
human	WB	Biochem Pharmacol; EYA4 reduces chemosensitivity of osteosarcoma to doxorubicin through DNA damage repair; Authors - Wei Heng
human	WB	Drug Resist Updat; RGS5+ lymphatic endothelial cells facilitate metastasis and acquired drug resistance of breast cancer through oxidative stress-sensing mechanism; Authors - Caixin Qiu