MRP3/ABCC3 Polyklonaler Antikörper

MRP3/ABCC3 Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 25358-1-PBS

Synonyme

ABCC3, MRP3, ABC31, ATP-binding cassette sub-family C member 3, EC:7.6.2.-

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  WB analysis using 25358-1-AP (same clone as 25358-1-PBS)

  37°C incubated HeLa cells were subjected to SDS PAGE followed by western blot with 25358-1-AP (MRP3/ABCC3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25358-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 25358-1-AP (same clone as 25358-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25358-1-AP (MRP3/ABCC3 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25358-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 25358-1-AP (same clone as 25358-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25358-1-AP (MRP3/ABCC3 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25358-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 25358-1-AP (same clone as 25358-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 25358-1-AP (MRP3/ABCC3 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25358-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

25358-1-PBS bindet in WB, IHC, Indirect ELISA MRP3/ABCC3 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MRP3/ABCC3 fusion protein Ag19786
• Vollständiger Name: ATP-binding cassette, sub-family C (CFTR/MRP), member 3
• Berechnetes Molekulargewicht: 1527 aa, 169 kDa
• Beobachtetes Molekulargewicht: 170-180 kDa
• GenBank-Zugangsnummer: BC137348
• Gene symbol: MRP3
• Gene ID (NCBI): 8714
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MRP3 also known as ABCC3, belongs to ATP-binding cassette (ABC) transporter protein superfamily. MRP3 hydrolyzes ATP to enable active transport of various substrates including many drugs, toxicants, and endogenous compounds across cell membranes (PMID: 11581266, 15083066). In normal liver, MRP3 was found present mainly in the bile ducts. In livers of patients with various forms of cholestasis, MRP3 levels were frequently increased in the proliferative cholangiocytes, suggesting its role in cholehepatic and enterohepatic bile circulation and in protecting liver from toxic bile salts( PMID: 11850532, 11283840). MRP3 is expressed in the liver, colon, pancreas, and, at a lower level, in the kidney (PMID:10094960). For optimal WB detection of this membrane protein, we recommend to avoid boiling the sample after lysis.