MRPL12 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

MRPL12 Polyklonal Antikörper für FC, IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, FC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

27

Kat-Nr. : 14795-1-AP

Synonyme

5c5 2, L12mt, MRP L12, MRP L31/34, MRPL12, MRPL7, MRPL7/L12, RPML12

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Galerie der Validierungsdaten

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  WB analysis using 14795-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14795-1-AP (MRPL12 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 14795-1-AP

  WB result of MRPL12 antibody (14795-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MRPL12 transfected HeLa cells.

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  IP experiment of HeLa using 14795-1-AP

  IP Result of anti-MRPL12 (IP:14795-1-AP, 4ug; Detection:14795-1-AP 1:500) with HeLa cells lysate 2000ug.

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  IHC staining of human colon cancer using 14795-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14795-1-AP (MRPL12 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 14795-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14795-1-AP (MRPL12 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 14795-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MRPL12 antibody (14795-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of HepG2 using 14795-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MRPL12 antibody (14795-1-AP) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of HepG2 using 14795-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MRPL12 antibody (14795-1-AP) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  FC experiment of HeLa using 14795-1-AP

  1X10^6 HeLa cells were stained with 0.2ug MRPL12 antibody (14795-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: COLO 320-Zellen, HeLa-Zellen, MCF-7-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:250-1:1000
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 25 publications below
• IHC: See 3 publications below
• IF: See 5 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

14795-1-AP bindet in WB, IP, IHC, IF, FC, CoIP, ELISA MRPL12 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MRPL12 fusion protein Ag6504
• Vollständiger Name: mitochondrial ribosomal protein L12
• Berechnetes Molekulargewicht: 21 kDa
• Beobachtetes Molekulargewicht: 21 kDa
• GenBank-Zugangsnummer: BC002344
• Gene symbol: MRPL12
• Gene ID (NCBI): 6182
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Mammalian MRPL12 was the first mitochondrial ribosomal protein to be characterized, and is encoded by a nuclear gene. The protein forms a homodimer, localizes predominantly to mitochondria and binds to the large mitochondrial ribosomal subunit [PMID:862670]. It is required for CycD/Cdk4-induced cell growth. In addition, forming a ribosomal stalk with MRPL7, MRPL12 is required for the recruitment of translation elongation factors Tu and G to the large ribosomal subunit [PMID:12370014]. It also a part of a novel regulatory mechanism that coordinates mitochondrial transcription with translation and/or ribosome biogenesis during human mitochondrial gene expression. The antibody reacts specifically with 21kDa MRPL12 protein.

Publikationen

Species	Application	Title
human	WB	Cell; An in vitro system to silence mitochondrial gene expression.; Authors - Luis Daniel Cruz-Zaragoza
human	WB	Nucleic Acids Res; The human Obg protein GTPBP10 is involved in mitoribosomal biogenesis.; Authors - Elena Lavdovskaia
human	WB	Nucleic Acids Res; METTL15 introduces N4-methylcytidine into human mitochondrial 12S rRNA and is required for mitoribosome biogenesis.; Authors - Lindsey Van Haute
human	WB	Genome Biol; Balanced mitochondrial and cytosolic translatomes underlie the biogenesis of human respiratory complexes; Authors - Iliana Soto
mouse	WB	EMBO Rep; Concerted regulation of mitochondrial and nuclear non-coding RNAs by a dual-targeted RNase Z.; Authors - Stefan J Siira
human	WB	EMBO Rep; Mitochondrial tyrosyl-DNA phosphodiesterase 2 and its TDP2Sshort isoform.; Authors - Shar-Yin N Huang