MRPL40 Rekombinanter Antikörper

MRPL40 Rekombinant Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

240506A9

Kat-Nr. : 83515-4-PBS

Synonyme

MRP-L40, MRP L40, MRP L22, Large ribosomal subunit protein mL40, L40mt

---

• 

  WB analysis using 83515-4-RR (same clone as 83515-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83515-4-RR (MRPL40 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83515-4-PBS in a different storage buffer formulation.

• 

  IHC staining of human intrahepatic cholangiocarcinoma using 83515-4-RR (same clone as 83515-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 83515-4-RR (MRPL40 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83515-4-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 83515-4-RR (same clone as 83515-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MRPL40 antibody (83515-4-RR, Clone: 240506A9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83515-4-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 83515-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83515-4-RR against Human MRPL40 were performed. The affinity constant is 1.56 nM.

---

Geprüfte Anwendungen

Produktinformation

83515-4-PBS bindet in WB, IHC, IF/ICC, ELISA MRPL40 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: MRPL40 fusion protein Ag10341
• Vollständiger Name: mitochondrial ribosomal protein L40
• Berechnetes Molekulargewicht: 206 aa, 24 kDa
• Beobachtetes Molekulargewicht: 18-24 kDa
• GenBank-Zugangsnummer: BC009707
• Gene symbol: MRPL40
• Gene ID (NCBI): 64976
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MRPL40, also known as NLVCF and URIM, belongs to the mitochondrion-specific ribosomal protein mL40 family. MRPL40 encodes the mitochondrial ribosomal protein L40 for the larger 39S subunit of mitochondrial ribosomes. MRPL40 is widely expressed in human organs and tissues, including the heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, ovary, small intestine, colon, peripheral blood lymphocyte (PBL), and testis. MRPL40 is also expressed in human fetuses and mouse embryos, suggesting its important role in fetal development (PMID: 36891938).