MRPL45 Polyklonaler Antikörper

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• KD/KO Validated

MRPL45 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

13

Kat-Nr. : 15682-1-AP

Synonyme

L45mt, MRPL45, MRP-L45

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Galerie der Validierungsdaten

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  WB analysis using 15682-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15682-1-AP (MRPL45 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15682-1-AP

  WB result of MRPL45 antibody (15682-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MRPL45 transfected HeLa cells.

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  WB analysis of mouse liver using 15682-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15682-1-AP (MRPL45 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 15682-1-AP

  IP result of anti-MRPL45(IP:15682-1-AP, 4ug; Detection:15682-1-AP 1:1000) with HeLa cells lysate 680 ug.

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  IHC staining of human ovary tumor using 15682-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 15682-1-AP (MRPL45 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 15682-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 15682-1-AP (MRPL45 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 15682-1-AP

  Immunofluorescent analysis of HeLa cells using 15682-1-AP (MRPL45 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Mauslebergewebe, SKOV-3-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Ovarialkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 11 publications below
• IF: See 2 publications below

Produktinformation

15682-1-AP bindet in WB, IP, IHC, IF, ELISA MRPL45 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MRPL45 fusion protein Ag8184
• Vollständiger Name: mitochondrial ribosomal protein L45
• Berechnetes Molekulargewicht: 35 kDa
• Beobachtetes Molekulargewicht: 35 kDa
• GenBank-Zugangsnummer: BC006235
• Gene symbol: MRPL45
• Gene ID (NCBI): 84311
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. MRPL45 is a 39S subunit protein.

Publikationen

Species	Application	Title
human	WB	Cell; An in vitro system to silence mitochondrial gene expression.; Authors - Luis Daniel Cruz-Zaragoza
mouse	WB	Sci Adv; Fidelity of translation initiation is required for coordinated respiratory complex assembly.; Authors - Danielle L Rudler
human	WB	Nat Commun; RNA modification landscape of the human mitochondrial tRNALys regulates protein synthesis.; Authors - Uwe Richter
human	IF	Proc Natl Acad Sci U S A; Pathological ribonuclease H1 causes R-loop depletion and aberrant DNA segregation in mitochondria.; Authors - Gokhan Akman
human	WB	J Cell Biol; FAM92A1 is a BAR domain protein required for mitochondrial ultrastructure and function.; Authors - Liang Wang
human	IF	Nucleic Acids Res; MPV17L2 is required for ribosome assembly in mitochondria.; Authors - Ilaria Dalla Rosa