MRPL45 Monoklonaler Antikörper

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MRPL45 Monoklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1B4B4

Kat-Nr. : 66531-1-PBS

Synonyme

L45mt, MRPL45, MRP-L45

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  WB analysis using 66531-1-Ig (same clone as 66531-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66531-1-Ig (MRPL45 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66531-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66531-1-Ig (same clone as 66531-1-PBS)

  WB result of MRPL45 antibody (66531-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MRPL45 transfected HeLa cells. This data was developed using the same antibody clone with 66531-1-PBS in a different storage buffer formulation.

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  WB analysis of HSC-T6 using 66531-1-Ig (same clone as 66531-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66531-1-Ig (MRPL45 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66531-1-PBS in a different storage buffer formulation.

• 

  WB analysis of RAW 264.7 using 66531-1-Ig (same clone as 66531-1-PBS)

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66531-1-Ig (MRPL45 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66531-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66531-1-Ig (same clone as 66531-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66531-1-Ig (MRPL45 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66531-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66531-1-Ig (same clone as 66531-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66531-1-Ig (MRPL45 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66531-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66531-1-PBS bindet in WB, IHC, Indirect ELISA MRPL45 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: MRPL45 fusion protein Ag8207
• Vollständiger Name: mitochondrial ribosomal protein L45
• Berechnetes Molekulargewicht: 35 kDa
• Beobachtetes Molekulargewicht: 33 kDa
• GenBank-Zugangsnummer: BC006235
• Gene symbol: MRPL45
• Gene ID (NCBI): 84311
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. MRPL45 is a 39S subunit protein.