MRPL49 Polyklonaler Antikörper

MRPL49 Polyklonal Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 15542-1-PBS

Synonyme

C11orf4, Large ribosomal subunit protein mL49, MRP-L49, NOF, NOF1

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  WB analysis of BxPC-3 using 15542-1-AP (same clone as 15542-1-PBS)

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 15542-1-AP (MRPL49 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15542-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 15542-1-AP (same clone as 15542-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 15542-1-AP (MRPL49 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15542-1-PBS in a different storage buffer formulation.

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  WB analysis of A549 using 15542-1-AP (same clone as 15542-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 15542-1-AP (MRPL49 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15542-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 15542-1-AP (same clone as 15542-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 15542-1-AP (MRPL49 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15542-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 15542-1-AP (same clone as 15542-1-PBS)

  IP result of anti-MRPL49 (IP:15542-1-AP, 4ug; Detection:15542-1-AP 1:500) with HeLa cells lysate 3360ug. This data was developed using the same antibody clone with 15542-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 15542-1-AP (same clone as 15542-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15542-1-AP (MRPL49 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15542-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 15542-1-AP (same clone as 15542-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15542-1-AP (MRPL49 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15542-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

15542-1-PBS bindet in WB, IHC, IP, Indirect ELISA MRPL49 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MRPL49 fusion protein Ag7898
• Vollständiger Name: mitochondrial ribosomal protein L49
• Berechnetes Molekulargewicht: 19 kDa
• Beobachtetes Molekulargewicht: 19 kDa
• GenBank-Zugangsnummer: BC004378
• Gene symbol: MRPL49
• Gene ID (NCBI): 740
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MRPL49, also named as C11orf4, NOF1, or SW-cl.67, is a 166 amino acid protein, which belongs to the ribosomal protein L49em family. MRPL49 localizes in the Mitochondrion and is widely expressed in various tissues. MRPL49 is a regulator of C3 convertase of classical pathway.