MRPL55 Polyklonaler Antikörper

MRPL55 Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Kat-Nr. : 17679-1-AP

Synonyme

MRP L55, Mitochondrial large ribosomal subunit protein bL31m, Large ribosomal subunit protein mL55, L55nt, L55mt

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  WB analysis of HEK-293 using 17679-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 17679-1-AP (MRPL55 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 17679-1-AP

  IP result of anti-MRPL55 (IP:17679-1-AP, 4ug; Detection:17679-1-AP 1:600) with HEK-293 cells lysate 1680 ug.

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  IP experiment of HEK-293 using 17679-1-AP

  IP result of anti-MRPL55 (IP:17679-1-AP, 4ug; Detection:17679-1-AP 1:800) with HEK-293 cells lysate 1640 ug.

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  IHC staining of human colon cancer using 17679-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 17679-1-AP (MRPL55 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 17679-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 17679-1-AP (MRPL55 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 17679-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17679-1-AP (MRPL55 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 17679-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17679-1-AP (MRPL55 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below

Produktinformation

17679-1-AP bindet in WB, IHC, IP, ELISA MRPL55 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MRPL55 fusion protein Ag11716
• Vollständiger Name: mitochondrial ribosomal protein L55
• Berechnetes Molekulargewicht: 128 aa, 15 kDa
• Beobachtetes Molekulargewicht: 13 kDa
• GenBank-Zugangsnummer: BC052806
• Gene symbol: MRPL55
• Gene ID (NCBI): 128308
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MRPL55, also named as L55mt and MRP-L55, is a component of the mitochondrial ribosome large subunit (39S). It acts dynamically in the cell during development. The MW of MRPL55 is about 13 kDa.

Publikationen

Species	Application	Title
human	WB	Cell Mol Life Sci; GTPBP8 is required for mitoribosomal biogenesis and mitochondrial translation; Authors - Liang Wang
human	WB	Mol Biol Cell; Ovarian carcinoma immunoreactive antigen-like protein 2 (OCIAD2) is a novel complex III-specific assembly factor in mitochondria.; Authors - Katarzyna Justyna Chojnacka
human,mouse	WB	Cell Rep; OCIAD1 and prohibitins regulate the stability of the TIM23 protein translocase; Authors - Praveenraj Elancheliyan
human	WB	Nat Struct Mol Biol; A roadmap for ribosome assembly in human mitochondria; Authors - Elena Lavdovskaia