MRPS22 Polyklonaler Antikörper

MRPS22 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

18

Kat-Nr. : 10984-1-AP

Synonyme

C3orf5, COXPD5, GIBT, GK002, MRP S22, MRPS22, RPMS22, S22mt

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Galerie der Validierungsdaten

• 

  WB analysis of Jurkat using 10984-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10984-1-AP (MRPS22 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 10984-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 10984-1-AP (MRPS22 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spleen using 10984-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 10984-1-AP (MRPS22 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat spleen using 10984-1-AP

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 10984-1-AP (MRPS22 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of Jurkat using 10984-1-AP

  IP Result of anti-MRPS22 (IP:10984-1-AP, 4ug; Detection:10984-1-AP 1:300) with Jurkat cells lysate 2400ug.

• 

  IHC staining of human breast cancer using 10984-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10984-1-AP (MRPS22 antibody) at dilution of 1:50 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, Mauslebergewebe, Mausmilzgewebe, Rattenmilzgewebe
• Erfolgreiche IP: Jurkat-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 18 publications below

Produktinformation

10984-1-AP bindet in WB, IP, IHC, ELISA MRPS22 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MRPS22 fusion protein Ag1421
• Vollständiger Name: mitochondrial ribosomal protein S22
• Berechnetes Molekulargewicht: 41 kDa
• Beobachtetes Molekulargewicht: 38 kDa
• GenBank-Zugangsnummer: BC009296
• Gene symbol: MRPS22
• Gene ID (NCBI): 56945
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MRPS22, also named as C3orf5, RPMS22 and S22mt, is a component of the mitochondrial ribosome small subunit (28S) which comprises a 12S rRNA and about 30 distinct proteins. Defects in MRPS22 are the cause of combined oxidative phosphorylation deficiency type 5 (COXPD5). MRPS22 is a 360-amino-acid protein that has been found in a limited number of species, having no ortholog in fungi or prokaryotes. The exact location of MRPS22 in the three dimensional structure of the ribosome and its exact function are currently unknown. Recently, a MRPS22 defect was shown to strongly hamper assembly of the small ribosomal subunit, whereas assembly of the large subunit and part of the small subunit were only mildly affected.(PMID:21189481)

Publikationen

Species	Application	Title
human	WB	Cell Metab; The mitochondrial RNA-binding protein GRSF1 localizes to RNA granules and is required for posttranscriptional mitochondrial gene expression.; Authors - Antonicka Hana H
human	WB	Cell Rep; The Human Mitochondrial DEAD-Box Protein DDX28 Resides in RNA Granules and Functions in Mitoribosome Assembly.; Authors - Ya-Ting Tu
human	WB	PLoS Genet; Mutations in the UQCC1-Interacting Protein, UQCC2, Cause Human Complex III Deficiency Associated with Perturbed Cytochrome b Protein Expression.; Authors - Elena J Tucker
human	WB	Nucleic Acids Res; C7orf30 specifically associates with the large subunit of the mitochondrial ribosome and is involved in translation.; Authors - Wanschers Bas F J BF
human, mouse	WB	Nucleic Acids Res; p32/gC1qR is indispensable for fetal development and mitochondrial translation: importance of its RNA-binding ability.; Authors - Yagi Mikako M
human	WB	Nucleic Acids Res; MPV17L2 is required for ribosome assembly in mitochondria.; Authors - Ilaria Dalla Rosa