MRPS27 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

MRPS27 Polyklonal Antikörper für IF, IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

26

Rezensionen (1)

Kat-Nr. : 17280-1-AP

Synonyme

KIAA0264, MRP S27, MRPS27, S27mt

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Galerie der Validierungsdaten

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  WB analysis of A431 using 17280-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 17280-1-AP (MRPS27 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human skeletal muscle using 17280-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 17280-1-AP (MRPS27 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 17280-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 17280-1-AP (MRPS27 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 17280-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 17280-1-AP (MRPS27 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of A431 using 17280-1-AP

  IP Result of anti-MRPS27 (IP:17280-1-AP, 3ug; Detection:17280-1-AP 1:1000) with A431 cells lysate 1520ug.

• 

  IHC staining of human kidney using 17280-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17280-1-AP (MRPS27 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human testis using 17280-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17280-1-AP (MRPS27 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 17280-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17280-1-AP (MRPS27 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human spleen using 17280-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17280-1-AP (MRPS27 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human spleen using 17280-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17280-1-AP (MRPS27 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human lung using 17280-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 17280-1-AP (MRPS27 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human lung using 17280-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 17280-1-AP (MRPS27 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human kidney using 17280-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17280-1-AP (MRPS27 antibody) at dilution of 1:100 (under 10x lens).

• 

  IF Staining of HepG2 using 17280-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MRPS27 rabbit poly (17280-1-AP, Green) and KRT18 mouse mAb (66187-1-Ig, Red) at dilution of 1:50, further stained with Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) for 17280-1-AP, and Alexa Fluor 594-conjugated AffiniPure Goat Anti-Mouse IgG (H+L) for 66187-1-Ig.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, humanes Herzgewebe, humanes Skelettmuskelgewebe
• Erfolgreiche IP: A431-Zellen
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Hodengewebe, humanes Lungengewebe, humanes Milzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 23 publications below
• IF: See 3 publications below

Produktinformation

17280-1-AP bindet in WB, IP, IHC, IF, ELISA MRPS27 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MRPS27 fusion protein Ag11047
• Vollständiger Name: mitochondrial ribosomal protein S27
• Berechnetes Molekulargewicht: 168 aa, 19 kDa, 48 kDa
• Beobachtetes Molekulargewicht: 48 kDa
• GenBank-Zugangsnummer: BC030521
• Gene symbol: MRPS27
• Gene ID (NCBI): 23107
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Genes encoding PPR (pentatricopeptide repeat)-containing proteins constitute one of the largest gene families in plants. The majority of these proteins are predicted to target organelles and to bind to RNA. Strikingly, there is a dearth of these proteins in mammals, although genomic searches reveal six candidates, all of which are also predicted to target the mitochondrion. One of these proteins, MRPS27is participated in transcription and translation respectively. [PMID:18939947] MRPS27is a component of the small subunit of the mitochondrial ribosome that is encoded by the nuclear genome [PMID: 11279123].

Publikationen

Species	Application	Title
human	WB	Cell Metab; The mitochondrial RNA-binding protein GRSF1 localizes to RNA granules and is required for posttranscriptional mitochondrial gene expression.; Authors - Antonicka Hana H
human	WB	Nat Commun; RNA modification landscape of the human mitochondrial tRNALys regulates protein synthesis.; Authors - Uwe Richter
mouse	WB	J Clin Invest; AFG3L2 supports mitochondrial protein synthesis and Purkinje cell survival.; Authors - Almajan Eva R ER
human	WB	Nucleic Acids Res; The human Obg protein GTPBP10 is involved in mitoribosomal biogenesis.; Authors - Elena Lavdovskaia
mouse	WB	J Cell Biol; Quality control of mitochondrial protein synthesis is required for membrane integrity and cell fitness.; Authors - Uwe Richter
human	WB	Proc Natl Acad Sci U S A; Mitochondrial ribosomal protein L12 selectively associates with human mitochondrial RNA polymerase to activate transcription.; Authors - Surovtseva Yulia V YV

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Xhuljana (Verified Customer) (03-01-2024)

WB in ripa lysate C2C12 cells

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: C2C12 cells

MRPS27 Antibody Western Blot validation (1:1000 dilution) in C2C12 cells (Cat no:17280-1-AP)