MRPS27 Monoklonaler Antikörper

MRPS27 Monoklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1B1B3

Kat-Nr. : 66724-1-PBS

Synonyme

FLJ21764, FLJ23348, KIAA0264, MRP S27, MRPS27, S27mt

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  WB analysis of LNCaP using 66724-1-Ig (same clone as 66724-1-PBS)

  LNCaP cells were subjected to SDS PAGE followed by western blot with 66724-1-Ig (MRPS27 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66724-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66724-1-Ig (same clone as 66724-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66724-1-Ig (MRPS27 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66724-1-PBS in a different storage buffer formulation.

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  WB analysis of A431 using 66724-1-Ig (same clone as 66724-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 66724-1-Ig (MRPS27 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66724-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 66724-1-Ig (same clone as 66724-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66724-1-Ig (MRPS27 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66724-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66724-1-Ig (same clone as 66724-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66724-1-Ig (MRPS27 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66724-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66724-1-Ig (same clone as 66724-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66724-1-Ig (MRPS27 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66724-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66724-1-PBS bindet in WB, IHC, Indirect ELISA MRPS27 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: MRPS27 fusion protein Ag11047
• Vollständiger Name: mitochondrial ribosomal protein S27
• Berechnetes Molekulargewicht: 168 aa, 19 kDa, 48 kDa
• Beobachtetes Molekulargewicht: 48 kDa
• GenBank-Zugangsnummer: BC030521
• Gene symbol: MRPS27
• Gene ID (NCBI): 23107
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Genes encoding PPR (pentatricopeptide repeat)-containing proteins constitute one of the largest gene families in plants. The majority of these proteins are predicted to target organelles and to bind to RNA. Strikingly, there is a dearth of these proteins in mammals, although genomic searches reveal six candidates, all of which are also predicted to target the mitochondrion. One of these proteins, MRPS27is participated in transcription and translation respectively. [PMID:18939947] MRPS27is a component of the small subunit of the mitochondrial ribosome that is encoded by the nuclear genome [PMID: 11279123].