MRPS35 Polyklonaler Antikörper

MRPS35 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 16457-1-PBS

Synonyme

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  WB analysis using 16457-1-AP (same clone as 16457-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 16457-1-AP (MRPS35 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16457-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 16457-1-AP (same clone as 16457-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 16457-1-AP (MRPS35 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16457-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human heart using 16457-1-AP (same clone as 16457-1-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 16457-1-AP (MRPS35 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16457-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HeLa using 16457-1-AP (same clone as 16457-1-PBS)

  IP result of anti-MRPS35 (IP:16457-1-AP, 4ug; Detection:16457-1-AP 1:1000) with HeLa cells lysate 1200ug. This data was developed using the same antibody clone with 16457-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 16457-1-AP (same clone as 16457-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16457-1-AP (MRPS35 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16457-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 16457-1-AP (same clone as 16457-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16457-1-AP (MRPS35 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16457-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 16457-1-AP (same clone as 16457-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using MRPS35 antibody (16457-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 16457-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

16457-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA MRPS35 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MRPS35 fusion protein Ag9430
• Vollständiger Name: mitochondrial ribosomal protein S35
• Berechnetes Molekulargewicht: 323 aa, 37 kDa
• Beobachtetes Molekulargewicht: 37 kDa
• GenBank-Zugangsnummer: BC015862
• Gene symbol: MRPS35
• Gene ID (NCBI): 60488
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Mammalian mitochondrial ribosomes (mitoribosomes) are responsible for protein synthesis within the mitochondrion. The mitoribosomes are composed of a 4:1 ratio of protein to RNA, with the proteins forming two subunits, the 28S subunit and the 39S subunit. Across species, the proteins that make up the mitoribosome subunits vary greatly in sequence, preventing easy recognition by sequence homology. MRPS35 (mitochondrial ribosomal protein S35), also known as MDS023, MRPS28 or HDCMD11P, is a 323 amino acid protein that localizes to the mitochondrion, where it exists as a component of the 28S ribosomal subunit and works in conjunction with other MRPs to mediate protein synthesis. Existing as two alternatively spliced isoforms, MRPS35 is encoded by a gene located on human chromosome 10, which houses over 1,200 genes and comprises nearly 4.5% of the human genome. This antibody is a rabbit polyclonal antibody raised against the full length MRPS35 protein. It specifically reacts with the 37kd MRPS35protein.