CD20 Polyklonaler Antikörper

CD20 Polyklonal Antikörper für FC, IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Rezensionen (1)

Kat-Nr. : 24828-1-AP

Synonyme

B lymphocyte antigen CD20, B1, Bp35, CD20, LEU 16, MS4A1, MS4A2, S7

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Galerie der Validierungsdaten

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  WB analysis using 24828-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24828-1-AP (CD20 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 24828-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 24828-1-AP (MS4A1,CD20 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of Raji using 24828-1-AP

  IP Result of anti-CD20 (IP:24828-1-AP, 4ug; Detection:24828-1-AP 1:600) with Raji cells lysate 1440ug.

• 

  IHC staining of human tonsillitis using 24828-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 24828-1-AP (CD20 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 24828-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 24828-1-AP (CD20 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 24828-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24828-1-AP (CD20 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 24828-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24828-1-AP (CD20 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 24828-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24828-1-AP (CD20 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 24828-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma slide using 24828-1-AP (MS4A1,CD20 Antibody) at dilution of 1:50.

• 

  IHC staining of human lymphoma using 24828-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma slide using 24828-1-AP (MS4A1,CD20 Antibody) at dilution of 1:50.

• 

  IHC staining of human tonsillitis using 24828-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis slide using 24828-1-AP (MS4A1,CD20 Antibody) at dilution of 1:50.

• 

  IHC staining of human tonsillitis using 24828-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis slide using 24828-1-AP (MS4A1,CD20 Antibody) at dilution of 1:50.

• 

  IHC staining of human colon cancer using 24828-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24828-1-AP (CD20 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 24828-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24828-1-AP (CD20 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 24828-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24828-1-AP (CD20 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human tonsillitis using 24828-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD20 antibody (24828-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CD3 antibody (60181-1-Ig, Clone: 3F3A1, red).

• 

  IF Staining of human tonsillitis using 24828-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD20 antibody (24828-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CD3 antibody (60181-1-Ig, Clone: 3F3A1, red).

• 

  IF Staining of Raji using 24828-1-AP

  Immunofluorescent analysis of (4% PFA) fixed Raji cells using CD20 antibody (24828-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human tonsillitis using 24828-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 24828-1-AP (CD20 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human tonsillitis using 24828-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 24828-1-AP (CD20 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human tonsillitis using 24828-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 24828-1-AP (CD20 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human tonsillitis using 24828-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD20 antibody (24828-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CD3 antibody (60181-1-Ig, Clone: 3F3A1, red).

• 

  IF Staining of human tonsillitis using 24828-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using PD-1/CD279 mouse mAb (66220-1-Ig) at dilution of 1:50 and CD20 rabbit pAb (24828-1-AP) at dilution of 1:50, further stained with Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L) for 66220-1-Ig, and Alexa Fluor 594-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) for 24828-1-AP.

• 

  IF Staining of human tonsillitis using 24828-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using PD-1/CD279 mouse mAb (66220-1-Ig) at dilution of 1:50 and CD20 rabbit pAb (24828-1-AP) at dilution of 1:50, further stained with Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L) for 66220-1-Ig, and Alexa Fluor 594-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) for 24828-1-AP.

• 

  FC experiment of Raji using 24828-1-AP

  1X10^6 Raji cells were stained with 0.2ug MS4A1,CD20 antibody (24828-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-Goat anti-Rabbit IgG with dilution 1:100.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Daudi-Zellen, Raji-Zellen, Ramos-Zellen
• Erfolgreiche IP: Raji-Zellen
• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe, humanes Kolonkarzinomgewebe, humanes Lymphomgewebe, humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: humanes Tonsillitisgewebe, Raji-Zellen
• Erfolgreiche Detektion in FC: Raji-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 3 publications below

Produktinformation

24828-1-AP bindet in WB, IP, IHC, IF, FC, ELISA CD20 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CD20 fusion protein Ag17309
• Vollständiger Name: membrane-spanning 4-domains, subfamily A, member 1
• Berechnetes Molekulargewicht: 297 aa, 33 kDa
• Beobachtetes Molekulargewicht: 35 kDa
• GenBank-Zugangsnummer: BC002807
• Gene symbol: MS4A1
• Gene ID (NCBI): 931
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD20 is a 33-37 kDa transmembrane phosphoprotein. CD20 is a B-lymphocyte surface molecule that is widely expressed during B-cell ontogeny, from early pre-B-cell developmental stages until final differentiation into plasma cells. CD20 functions as calcium-permeable cation channel. It is involved in the regulation of B-cell activation and proliferation. CD20 serves as a useful target for antibody-mediated therapeutic depletion of B-cells.

Publikationen

Species	Application	Title
human	IHC	J Immunother Cancer; Infiltrating pattern and prognostic value of tertiary lymphoid structures in resected non-functional pancreatic neuroendocrine tumors.; Authors - Wu-Hu Zhang
human	IHC	Clin Exp Immunol; CMTM6: increased circulating level and up-regulated expression in labial salivary glands in patients with primary Sjogren's syndrome.; Authors - Sirui Qian
human	WB	Acta Physiol (Oxf); Acute physical exercise and long-term individual shear rate therapy increase telomerase activity in human peripheral blood mononuclear cells.; Authors - A Zietzer
human	IHC	Int J Mol Sci; CXCR4 Expressed by Tumor-Infiltrating B Cells in Gastric Cancer Related to Survival in the Tumor Microenvironment: An Analysis Combining Single-Cell RNA Sequencing with Bulk RNA Sequencing; Authors - Chen Su

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Emma (Verified Customer) (11-29-2021)

Works well by IF on FFPE tissue. Antigen retrieval Tris-EDTA pH 9. Also works by IHC with sodium citrate antigen retrieval.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:50
• Cell Tissue Type: Prostate

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