MSH2 Rekombinanter Antikörper

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MSH2 Rekombinant Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

6O4

Kat-Nr. : 82775-1-PBS

Synonyme

MSH 2, 6O4

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• 

  WB analysis using 82775-1-RR (same clone as 82775-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82775-1-RR (MSH2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82775-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 82775-1-RR (same clone as 82775-1-PBS)

  WB result of MSH2 antibody (82775-1-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MSH2 transfected HeLa cells. This data was developed using the same antibody clone with 82775-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 82775-1-RR (same clone as 82775-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 82775-1-RR (MSH2 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82775-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 82775-1-RR (same clone as 82775-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 82775-1-RR (MSH2 antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82775-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 82775-1-RR (same clone as 82775-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 82775-1-RR (MSH2 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82775-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 82775-1-RR (same clone as 82775-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 82775-1-RR (MSH2 antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82775-1-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 82775-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82775-1-RR against Human MSH2 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

Produktinformation

82775-1-PBS bindet in WB, IHC, Indirect ELISA MSH2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: MSH2 fusion protein Ag7835
• Vollständiger Name: mutS homolog 2, colon cancer, nonpolyposis type 1 (E. coli)
• Berechnetes Molekulargewicht: 105 kDa
• Beobachtetes Molekulargewicht: 100 kDa-105 kDa
• GenBank-Zugangsnummer: BC021566
• Gene symbol: MSH2
• Gene ID (NCBI): 4436
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MSH2, named for its homologous to the E.coli MutS gene, takes part in DNA mismatch repair(MMR) to maintain the stability of DNA. MSH2 contains a helix-turn-helix domain, which responds for binding to DNA. Also, MSH2 has a direct role in mutation avoidance and micro-satellite stability in cell.