MSH3 Rekombinanter Antikörper

• Featured Product
• KD/KO Validated

MSH3 Rekombinant Antikörper für WB, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Ratte

Anwendung

WB, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

CloneNo.

1J23

Kat-Nr. : 82845-3-RR

Synonyme

1J23, Divergent upstream protein, DUC1, DUG, hMSH3

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  WB analysis using 82845-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82845-3-RR (MSH3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 82845-3-RR

  WB result of MSH3 antibody (82845-3-RR; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MSH3 transfected HEK-293 cells.

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  IP experiment of HeLa using 82845-3-RR

  IP result of anti-MSH3 (IP:82845-3-RR, 4ug; Detection:82845-3-RR 1:3000) with HeLa cells lysate 1320 ug.

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  IF Staining of U-251 using 82845-3-RR

  Immunofluorescent analysis of (4% PFA) fixed U-251 cells using MSH3 antibody (82845-3-RR, Clone: 1J23 ) at dilution of 1:1500 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of U2OS using 82845-3-RR

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using MSH3 antibody (82845-3-RR, Clone: 1J23 ) at dilution of 1:1500 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

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  Affinity and Kinetic Characterization of 82845-3-RR

  Biolayer interferometry (BLl) kinetic assays of 82845-3-RR against Human MSH3 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HSC-T6 cells, HEK-293-Zellen, HEK-293-Zellen, HeLa-Zellen, MOLT-4-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IF/ICC: U-251-Zellen, U2OS-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:750-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

82845-3-RR bindet in WB, IF/ICC, IP, ELISA MSH3 und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: MSH3 fusion protein Ag18060
• Vollständiger Name: mutS homolog 3 (E. coli)
• Berechnetes Molekulargewicht: 1137 aa, 127 kDa
• Beobachtetes Molekulargewicht: 127 kDa
• GenBank-Zugangsnummer: BC130434
• Gene symbol: MSH3
• Gene ID (NCBI): 4437
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MSH3 is a DNA mismatch repair (MMR) gene that heterodimerizes with MSH2 to form MutS beta which binds to DNA mismatches thereby initiating DNA repair. It undergoes frequent somatic mutation in colorectal cancers (CRCs) with MMR deficiency. MSH3 mismatch repair protein regulates sensitivity to cytotoxic drugs and a histone deacetylase inhibitor in human colon carcinoma cells (PMID: 23724141, PMID:32671096).