MSI1 Rekombinanter Antikörper

MSI1 Rekombinant Antikörper für WB, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

251695G8

Kat-Nr. : 86723-1-PBS

Synonyme

Musashi 1, musashi homolog 1 (Drosophila), Musashi-1, RNA-binding protein Musashi homolog 1

---

• 

  WB analysis using 86723-1-RR (same clone as 86723-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86723-1-RR (MSI1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86723-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 86723-1-RR (same clone as 86723-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86723-1-RR (MSI1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86723-1-PBS in a different storage buffer formulation.

• 

  Cytometric bead array sample test of MP02619-1

  The mean MSI1 concentration was determined to be 12.9 ng/mL in HEK-293 cell extract based on a 1.2 mg/mL extract load, 9.0 ng/mL in Caco-2 cell extract based on a 1.3 mg/mL extract load, 20.7 ng/mL in HEK-293T cell extract based on a 2.6 mg/mL extract load.

• 

  Cytometric bead array standard curve of MP02619-1

  Cytometric bead array standard curve of MP02619-1, MSI1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 86723-1-PBS. Detection antibody: 86723-2-PBS. Standard: Ag26042. Range: 0.312-40 ng/mL.

• 

  Affinity and Kinetic Characterization of 86723-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86723-1-RR against Human MSI1 were performed. The affinity constant is 93.8 pM.

---

Geprüfte Anwendungen

Produktinformation

86723-1-PBS bindet in WB, Cytometric bead array, Indirect ELISA MSI1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: MSI1 fusion protein Ag26042
• Vollständiger Name: musashi homolog 1 (Drosophila)
• Berechnetes Molekulargewicht: 362 aa, 39 kDa
• Beobachtetes Molekulargewicht: 37-40 kDa
• GenBank-Zugangsnummer: BC146463
• Gene symbol: MSI1
• Gene ID (NCBI): 4440
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Musashi1 (Msi1) is an RNA-binding protein expressed in neural progenitor cells and neural stem cells. The gene encoding human Msi1 encodes a 362 amino acid protein. In murine embryonic neural progenitor cells, Msi1 localizes to the cytoplasm and is downregulated during differentiation. Msi1 binds to NUMB, which encodes a membrane-associated antagonist of Notch signaling. Msi1 appears to function in the proliferation and maintenance of stem cell populations of the central nervous system. In addition to its usefulness as a marker for neural progenitor cells in normal human brains, Msi1 is also a marker for human gliomas. In rats, Msi1 is expressed in Sertoli cells of the testis and granulosa cells of the ovary.