MSI2 Polyklonaler Antikörper

MSI2 Polyklonal Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 10770-1-PBS

Synonyme

Musashi-2, RNA-binding protein Musashi homolog 2, Musashi 2, MSI2H

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  WB analysis of PC-12 using 10770-1-AP (same clone as 10770-1-PBS)

  PC-12 cells were subjected to SDS PAGE followed by western blot with 10770-1-AP (MSI2 antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10770-1-PBS in a different storage buffer formulation.

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  WB analysis of COLO 320 using 10770-1-AP (same clone as 10770-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 10770-1-AP (MSI2 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10770-1-PBS in a different storage buffer formulation.

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  WB analysis of C6 using 10770-1-AP (same clone as 10770-1-PBS)

  C6 cells were subjected to SDS PAGE followed by western blot with 10770-1-AP (MSI2 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10770-1-PBS in a different storage buffer formulation.

• 

  WB analysis of C6 using 10770-1-AP (same clone as 10770-1-PBS)

  C6 cells were subjected to SDS PAGE followed by western blot with 10770-1-AP (MSI2 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10770-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 10770-1-AP (same clone as 10770-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 10770-1-AP (MSI2 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10770-1-PBS in a different storage buffer formulation.

• 

  WB analysis of PC-12 using 10770-1-AP (same clone as 10770-1-PBS)

  PC-12 cells were subjected to SDS PAGE followed by western blot with 10770-1-AP (MSI2 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10770-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 10770-1-AP (same clone as 10770-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 10770-1-AP (MSI2 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10770-1-PBS in a different storage buffer formulation.

• 

  WB analysis of PC-12 using 10770-1-AP (same clone as 10770-1-PBS)

  PC-12 cells were subjected to SDS PAGE followed by western blot with 10770-1-AP (MSI2 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10770-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 10770-1-AP (same clone as 10770-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 10770-1-AP (MSI2 antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10770-1-PBS in a different storage buffer formulation.

• 

  IP experiment of MCF-7 using 10770-1-AP (same clone as 10770-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 10770-1-AP (MSI2 antibody) at dilution of 1:500. This data was developed using the same antibody clone with 10770-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary tumor using 10770-1-AP (same clone as 10770-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10770-1-AP (MSI2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10770-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary tumor using 10770-1-AP (same clone as 10770-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10770-1-AP (MSI2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10770-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 10770-1-AP (same clone as 10770-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 10770-1-AP (MSI2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10770-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 10770-1-AP (same clone as 10770-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10770-1-AP (MSI2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10770-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 10770-1-AP (same clone as 10770-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 10770-1-AP (MSI2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10770-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 10770-1-AP (same clone as 10770-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10770-1-AP (MSI2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10770-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

10770-1-PBS bindet in WB, IHC, IP, Indirect ELISA MSI2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MSI2 fusion protein Ag1213
• Vollständiger Name: musashi homolog 2 (Drosophila)
• Berechnetes Molekulargewicht: 35 kDa
• Beobachtetes Molekulargewicht: 37-40 kDa
• GenBank-Zugangsnummer: BC001526
• Gene symbol: MSI2
• Gene ID (NCBI): 124540
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MSI2 is an RNA-binding protein that regulates the expression of target mRNAs at the translation level. It binds to RNA via 2 ribonucleoprotein-type RNA recognition motifs in their N-termini, to consensus motifs in mRNAs to inhibit transcription. It may play a role in the proliferation and maintenance of stem cells in the central nervous system. MSI2 can regulate normal hematopoiesis and promote aggressive myeloid leukemia