Mesothelin Rekombinanter Antikörper

• Featured Product
• KD/KO Validated

Mesothelin Rekombinant Antikörper für WB, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

240795G5

Kat-Nr. : 83744-2-PBS

Synonyme

MSLN, 240795G5, CAK1, CAK1 antigen, Megakaryocyte-potentiating factor

---

• 

  WB analysis using 83744-2-RR (same clone as 83744-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83744-2-RR (MSLN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83744-2-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 83744-2-RR (same clone as 83744-2-PBS)

  WB result of Mesothelin antibody (83744-2-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Mesothelin transfected HeLa cells. This data was developed using the same antibody clone with 83744-2-PBS in a different storage buffer formulation.

• 

  Sandwich ELISA standard curve of MP00697-2

  Sandwich ELISA standard curve of MP00697-2, Human MSLN/Mesothelin Recombinant Matched Antibody Pair - PBS only. 83744-5-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg31658. 83744-2-PBS was HRP conjugated as the detection antibody. Range: 39.1-2500 pg/mL

• 

  Sample test of MP00697-2

  Urine of four humans was measured. The human MSLN/Mesothelin concentration of detected samples was determined to be 923.5 pg/mL with a range of 138.7 - 1,373.8 pg/mL.

• 

  Sample test of MP00697-2

  Saliva of four humans was measured. The human MSLN/Mesothelin concentration of detected samples was determined to be 5,273.4 pg/mL with a range of 209.0 - 16,634.9 pg/mL.

• 

  Sample test of MP00697-2

  Serum of four humans was measured. The human MSLN/Mesothelin concentration of detected samples was determined to be 35,341.8 pg/mL with a range of 16,291.9 - 52,246.0 pg/mL.

• 

  IF Staining of HeLa using 83744-2-RR (same clone as 83744-2-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using MSLN antibody (83744-2-RR, Clone: 240795G5 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83744-2-PBS in a different storage buffer formulation.

• 

  IF Staining of THP-1 using 83744-2-RR (same clone as 83744-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using MSLN antibody (83744-2-RR, Clone: 240795G5 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83744-2-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP00697-2

  Cytometric bead array standard curve of MP00697-2, MSLN/Mesothelin Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83744-5-PBS. Detection antibody: 83744-2-PBS. Standard: Eg31658. Range: 0.625-80 ng/mL.

• 

  Affinity and Kinetic Characterization of 83744-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83744-2-RR against Human MSLN were performed. The affinity constant is 1.03 nM.

---

Geprüfte Anwendungen

Produktinformation

83744-2-PBS bindet in WB, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Mesothelin und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Mesothelin fusion protein Eg31658
• Vollständiger Name: mesothelin
• Berechnetes Molekulargewicht: 69KD
• Beobachtetes Molekulargewicht: 69 kDa, 40 kDa
• GenBank-Zugangsnummer: NM_005823
• Gene symbol: MSLN
• Gene ID (NCBI): 10232
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Mesothelin (MSLN) is a 40kDa GPI-anchored surface glycoprotein that is expressed on pleural and peritoneal mesothelial cells, but not in other non-neoplastic tissues. The mesothelin gene encodes a 69-71kDa precursor protein that is processed to a 40kDa membrane-bound protein termed mesothelin and a 31kDa shed fragment called megakaryocyte-potentiating factor (MPF) that is released from the cell. Mesothelin is a tumor differentiation antigen present at low levels on a restricted set of normal adult tissues (e.g. mesothelium), and is expressed at high levels in mesotheliomas, as well as ovarian, pancreatic, and lung cancers. Mesothelin may be a new target for immunotherapy (PMID: 20922056, 18691948, 15217923).