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MSMP Polyklonaler Antikörper

MSMP Polyklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 19526-1-PBS

Synonyme

Filter:
  • Western Blot (WB) analysis of various lysates using MSMP Polyclonal antibody (19526-1-AP)

    WB analysis using 19526-1-AP (same clone as 19526-1-PBS)

    PC-3 cells were subjected to SDS PAGE followed by western blot with 19526-1-AP (MSMP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19526-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of DU 145 cells using MSMP Polyclonal antibody (19526-1-AP)

    WB analysis of DU 145 using 19526-1-AP (same clone as 19526-1-PBS)

    WB result of MSMP antibody (19526-1-AP; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-MSMP transfected DU 145 cells. This data was developed using the same antibody clone with 19526-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using MSMP Polyclonal antibody (19526-1-AP)

    WB analysis using 19526-1-AP (same clone as 19526-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 19526-1-AP (MSMP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19526-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of PC-3 cells using MSMP Polyclonal antibody (19526-1-AP)

    IF Staining of PC-3 using 19526-1-AP (same clone as 19526-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed PC-3 cells using MSMP antibody (19526-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 19526-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

19526-1-PBS bindet in WB, IF/ICC, Indirect ELISA MSMP und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen Peptid
Vollständiger Name microseminoprotein, prostate associated
Berechnetes Molekulargewicht 15 kDa
Beobachtetes Molekulargewicht12 kDa, 20 kDa
GenBank-ZugangsnummerNM_001044264
Gene symbol MSMP
Gene ID (NCBI) 692094
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MSMP, also named PSMP, belongs to the beta-microseminoprotein family. MSMP is highly conserved, sharing 91 to 94% amino acid identity with its rodent and dog homologs. RT-PCR of 27 human tissues detected PSMP expression only in trachea and, to a lesser extent, testis. MSMP was highly expressed in PC3 cells, but not in 2 other prostate cancer cell lines. Western blot analysis revealed 20 kDa and 12 kDa bands PSMP maybe due to posttranslational modification.