MSRA Polyklonaler Antikörper

MSRA Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 14547-1-PBS

Synonyme

---

• 

  WB analysis of human kidney using 14547-1-AP (same clone as 14547-1-PBS)

  human kidney tissue were subjected to SDS PAGE followed by western blot with 14547-1-AP (MSRA antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14547-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 14547-1-AP (same clone as 14547-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14547-1-AP (MSRA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14547-1-PBS in a different storage buffer formulation.

• 

  IP experiment of mouse kidney using 14547-1-AP (same clone as 14547-1-PBS)

  IP result of anti-MSRA (IP:14547-1-AP, 4ug; Detection:14547-1-AP 1:600) with mouse kidney tissue lysate 4000ug. This data was developed using the same antibody clone with 14547-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 14547-1-AP (same clone as 14547-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14547-1-AP (MSRA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14547-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 14547-1-AP (same clone as 14547-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14547-1-AP (MSRA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14547-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HEK-293 using 14547-1-AP (same clone as 14547-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using 14547-1-AP (MSRA antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14547-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

14547-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA MSRA und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MSRA fusion protein Ag6053
• Vollständiger Name: methionine sulfoxide reductase A
• Berechnetes Molekulargewicht: 26 kDa
• Beobachtetes Molekulargewicht: 24-26 kDa
• GenBank-Zugangsnummer: BC054033
• Gene symbol: MSRA
• Gene ID (NCBI): 4482
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Methionine sulfoxide reductase A(MSRA), is a ubiquitous and highly conserved protein that carries out the enzymatic reduction of methionine sulfoxide to methionine.MSRA has some isoforms with MW of 19~26 kDa. MSRA functions in the repair of oxidatively damaged proteins to restore biological activity. Transfection of MSRA reduced colony formation and the invasiveness of HCC cell lines.