MSRA Rekombinanter Antikörper

MSRA Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Ratte

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

251025H2

Kat-Nr. : 86358-1-RR

Synonyme

EC:1.8.4.11, Mitochondrial peptide methionine sulfoxide reductase, Peptide Met(O) reductase, Peptide-methionine, PMSR

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  WB analysis using 86358-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86358-1-RR (MSRA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of THP-1 using 86358-1-RR

  Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using MSRA antibody (86358-1-RR, Clone: 251025H2 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: THP-1-Zellen, HT-1080.Zellen, Rattennierengewebe
• Erfolgreiche Detektion in IF/ICC: THP-1-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

86358-1-RR bindet in WB, IF/ICC, ELISA MSRA und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: MSRA fusion protein Ag6053
• Vollständiger Name: methionine sulfoxide reductase A
• Berechnetes Molekulargewicht: 26 kDa
• Beobachtetes Molekulargewicht: 24-26 kDa
• GenBank-Zugangsnummer: BC054033
• Gene symbol: MSRA
• Gene ID (NCBI): 4482
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Methionine sulfoxide reductase A (MSRA) is an antioxidant enzyme found in all domains of life that catalyzes the reduction of methionine-S-sulfoxide (MSO) to methionine in proteins and free amino acids (PMID: 28874471). MSRA has some isoforms with MW of 19~26 kDa. MSRA functions in the repair of oxidatively damaged proteins to restore biological activity. Transfection of MSRA reduced colony formation and the invasiveness of HCC cell lines. Studies have demonstrated the downregulation of MSRA in multiple human tumors, including breast cancers and its levels have been linked to advanced tumor grades (PMID: 20937881).