MT1X Polyklonaler Antikörper

MT1X Polyklonal Antikörper für IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (2)

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Rezensionen (1)

Kat-Nr. : 17172-1-AP

Synonyme

metallothionein 1X, Metallothionein IX, MT 1l, MT 1X, MT IX, MT1, MT1X

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Galerie der Validierungsdaten

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  IHC staining of human hepatocirrhosis using 17172-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 17172-1-AP (MT1X antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 17172-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17172-1-AP (MT1X antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver using 17172-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17172-1-AP (MT1X antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human hepatocirrhosis using 17172-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 17172-1-AP (MT1X antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: humanes Lebergewebe, humanes Leberzirrhosegewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 5 publications below

Produktinformation

17172-1-AP bindet in WB, IHC, ELISA MT1X und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Huhn, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MT1X fusion protein Ag10711
• Vollständiger Name: metallothionein 1X
• Berechnetes Molekulargewicht: 46 aa, 5 kDa
• GenBank-Zugangsnummer: BC032131
• Gene symbol: MT1X
• Gene ID (NCBI): 4501
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human,mouse	WB	Autophagy; Therapeutic targeting of the USP2-E2F4 axis inhibits autophagic machinery essential for zinc homeostasis in cancer progression.; Authors - Wenjing Xiao
human	IHC	Cancer Res; Intratumoral Copper Modulates PD-L1 Expression and Influences Tumor Immune Evasion.; Authors - Florida Voli
human	IHC	J Cell Mol Med; Comprehensive analysis of partial epithelial mesenchymal transition-related genes in hepatocellular carcinoma.; Authors - Yu Lei
human	IHC	Cancer Biol Ther; MiR-376a-3p increases cell apoptosis in acute myeloid leukemia by targeting MT1X.; Authors - Xiangke Xin
human	IHC	Cell Death Dis; Comparison of the major cell populations among osteoarthritis, Kashin-Beck disease and healthy chondrocytes by single-cell RNA-seq analysis.; Authors - Xi Wang
chicken	IHC	Poult Sci; The capability of L-carnitine-mediated antioxidant on cock during aging: evidence for the improved semen quality and enhanced testicular expressions of GnRH1, GnRHR, and melatonin receptors MT 1/2.; Authors - Abdelmotaleb A Elokil

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Adrianna (Verified Customer) (10-31-2022)

There is a lot of unspecific bands with this antibody and I cannot see the actual band being brighter. I have used it at 1:1000 dilution, and incubated ON at 4 deg C. Perhaps I should try a different dilution / lysate concentration.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: MCF7