MTA2 Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

MTA2 Monoklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Konjugation

Unkonjugiert

Publikationen(8)

CloneNo.

2G12G4

Kat-Nr. : 66195-1-Ig

Synonyme

2G12G4, Metastasis associated 1 like 1, Metastasis-associated 1-like 1, Metastasis-associated protein MTA2, MTA1 L1 protein

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  WB analysis using 66195-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66195-1-Ig (MTA2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 66195-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66195-1-Ig (MTA2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 66195-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66195-1-Ig (MTA2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse lung using 66195-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 66195-1-Ig (MTA2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung using 66195-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 66195-1-Ig (MTA2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 66195-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using MTA2 antibody (66195-1-Ig, Clone: 2G12G4 ) at dilution of 1:2000 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-Phalloidin (red).

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  FC experiment of HepG2 using 66195-1-Ig

  1X10^6 HepG2 cells were stained with .2ug MTA2 antibody (66195-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1000.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: SW480-Zellen, 4T1-Zellen, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, K-562-Zellen, NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: Maushirngewebe, humanes Lungengewebe, Mauslungengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: A431-Zellen
• Erfolgreiche Detektion in FC (Intra): HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:1000-1:2000
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.2 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 6 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Produktinformation

66195-1-Ig bindet in WB, IHC, IF/ICC, FC (Intra), IP, ELISA MTA2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: MTA2 fusion protein Ag11713
• Vollständiger Name: metastasis associated 1 family, member 2
• Berechnetes Molekulargewicht: 668 aa, 75 kDa
• Beobachtetes Molekulargewicht: 75 kDa
• GenBank-Zugangsnummer: BC053650
• Gene symbol: MTA2
• Gene ID (NCBI): 9219
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Metastasis-associated protein MTA2, also named as MTA1L1 or PID, is a 668 amino acid protein, which contains one BAH domain, one ELM2 domain, one GATA-type zinc finger and one SANT domain. MTA2 localizes in the nucleus and is widely expressed in many tissues. MTA2 may be involved in the regulation of gene expression as repressor and activator.

Publikationen

Species	Application	Title
		Dev Cell; Nucleoporin Seh1 controls murine neocortical development via transcriptional repression of p21 in neural stem cells; Authors - Wenxiu Dai
human	WB,IF	Theranostics; LncRNA-MTA2TR functions as a promoter in pancreatic cancer via driving deacetylation-dependent accumulation of HIF-1α.; Authors - Zhu Zeng ; KD Validated
Mouse	IF	Cell Mol Gastroenterol Hepatol; Targeting HNRNPM Inhibits Cancer Stemness and Enhances Antitumor Immunity in Wnt-activated Hepatocellular Carcinoma.; Authors - Gui-Qi Zhu
human	WB	Oncol Lett; Demethylation of HACE1 gene promoter by propofol promotes autophagy of human A549 cells.; Authors - Shanshan Li
human	WB	Cancers (Basel); ZEB2/TWIST1/PRMT5/NuRD Multicomplex Contributes to the Epigenetic Regulation of EMT and Metastasis in Colorectal Carcinoma; Authors - Yayuan Zheng
human	WB	Nat Chem Biol; ENO1 promotes liver carcinogenesis through YAP1-dependent arachidonic acid metabolism; Authors - Linchong Sun ; KD Validated