MTA3 Rekombinanter Antikörper

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• KD/KO Validated

MTA3 Rekombinant Antikörper für WB, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

230350D12

Kat-Nr. : 83042-3-PBS

Synonyme

230350D12, Metastasis-associated protein MTA3, MTA 3, mta1

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  WB analysis using 83042-3-RR (same clone as 83042-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83042-3-RR (MTA3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83042-3-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 83042-3-RR (same clone as 83042-3-PBS)

  WB result of MTA3 antibody (83042-3-RR; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MTA3 transfected MCF-7 cells. This data was developed using the same antibody clone with 83042-3-PBS in a different storage buffer formulation.

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  Sandwich ELISA sample test of MP00017-2

  Hela cell lysate was measured. The human MTA3 concentration of detected samples was determined to be 50.22 ng/mL (based on a 3.2 mg/mL extract load).

• 

  Sandwich ELISA standard curve of MP00017-2

  Sandwich ELISA standard curve of MP00017-2, Human MTA3 Recombinant Matched Antibody Pair - PBS only. 83042-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag6400. 83042-1-PBS was HRP conjugated as the detection antibody. Range: 3.13-200 ng/mL

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  IF Staining of U-251 using 83042-3-RR (same clone as 83042-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U-251 cells using MTA3 antibody (83042-3-RR, Clone: 230350D12 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83042-3-PBS in a different storage buffer formulation.

• 

  FC experiment of U-251 using 83042-3-RR (same clone as 83042-3-PBS)

  1x10^6 U-251 cells were intracellularly stained with 0.25 ug MTA3 Recombinant antibody (83042-3-RR, Clone:230350D12) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 83042-3-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP00017-2

  Cytometric bead array standard curve of MP00017-2, MTA3 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83042-3-PBS. Detection antibody: 83042-1-PBS. Standard: Ag6400. Range: 0.625-20 ng/mL.

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  Affinity and Kinetic Characterization of 83042-3-PBS

  Biolayer interferometry (BLl) kinetic assay of 83042-3-PBS against Human MTA3 was performed. The affinity constant is below 1 pM.

• 

  Affinity and Kinetic Characterization of 83042-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83042-3-RR against Human MTA3 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

Produktinformation

83042-3-PBS bindet in WB, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA MTA3 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: MTA3 fusion protein Ag6400
• Vollständiger Name: metastasis associated 1 family, member 3
• Berechnetes Molekulargewicht: 68 kDa
• Beobachtetes Molekulargewicht: 62 kDa, 68 kDa
• GenBank-Zugangsnummer: BC053631
• Gene symbol: MTA3
• Gene ID (NCBI): 57504
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The metastasis-associated (MTA) family contains three members, MTA1, MTA2 and MTA3 (PMID:12167865).Metastasis-associated protein 3 (MTA3) is a cell-type specific component of the Mi-2-NURD transcriptional co-repressor complex. It has a role in maintenance of the normal epithelial architecture through the repression of SNAI1 transcription in a histone deacetylase-dependent manner, and thus the regulation of E-cadherin levels.