MTA3 Rekombinanter Antikörper

• Featured Product
• KD/KO Validated

MTA3 Rekombinant Antikörper für WB, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

230350D12

Kat-Nr. : 83042-3-RR

Synonyme

230350D12, Metastasis-associated protein MTA3, MTA 3, mta1

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  WB analysis using 83042-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83042-3-RR (MTA3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 83042-3-RR

  WB result of MTA3 antibody (83042-3-RR; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MTA3 transfected MCF-7 cells.

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  IF Staining of U-251 using 83042-3-RR

  Immunofluorescent analysis of (4% PFA) fixed U-251 cells using MTA3 antibody (83042-3-RR, Clone: 230350D12 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

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  FC experiment of U-251 using 83042-3-RR

  1x10^6 U-251 cells were intracellularly stained with 0.25 ug MTA3 Recombinant antibody (83042-3-RR, Clone:230350D12) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

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  Affinity and Kinetic Characterization of 83042-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83042-3-RR against Human MTA3 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, A375-Zellen, C2C12-Zellen, MCF-7-Zellen, NIH/3T3-Zellen, Raji-Zellen
• Erfolgreiche Detektion in IF/ICC: U-251-Zellen
• Erfolgreiche Detektion in FC (Intra): U-251-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83042-3-RR bindet in WB, IF/ICC, FC (Intra), ELISA MTA3 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: MTA3 fusion protein Ag6400
• Vollständiger Name: metastasis associated 1 family, member 3
• Berechnetes Molekulargewicht: 68 kDa
• Beobachtetes Molekulargewicht: 62 kDa, 68 kDa
• GenBank-Zugangsnummer: BC053631
• Gene symbol: MTA3
• Gene ID (NCBI): 57504
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The metastasis-associated (MTA) family contains three members, MTA1, MTA2 and MTA3 (PMID:12167865).Metastasis-associated protein 3 (MTA3) is a cell-type specific component of the Mi-2-NURD transcriptional co-repressor complex. It has a role in maintenance of the normal epithelial architecture through the repression of SNAI1 transcription in a histone deacetylase-dependent manner, and thus the regulation of E-cadherin levels.