MTAP Monoklonaler Antikörper

MTAP Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

CloneNo.

2B1G6

Kat-Nr. : 66706-1-Ig

Synonyme

c86fus, MSAP, MTA phosphorylase, MTAP, MTAPase

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Galerie der Validierungsdaten

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  WB analysis of NIH/3T3 using 66706-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66706-1-Ig (MTAP antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 66706-1-Ig

  Raji cells were subjected to SDS PAGE followed by western blot with 66706-1-Ig (MTAP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HT-29 using 66706-1-Ig

  HT-29 cells were subjected to SDS PAGE followed by western blot with 66706-1-Ig (MTAP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HCT 116 using 66706-1-Ig

  HCT 116 cells were subjected to SDS PAGE followed by western blot with 66706-1-Ig (MTAP antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HSC-T6 using 66706-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66706-1-Ig (MTAP antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse liver using 66706-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66706-1-Ig (MTAP antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 66706-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66706-1-Ig (MTAP antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 66706-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66706-1-Ig (MTAP antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 66706-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66706-1-Ig (MTAP antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 66706-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66706-1-Ig (MTAP antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 66706-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66706-1-Ig (MTAP antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 66706-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66706-1-Ig (MTAP antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 66706-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66706-1-Ig (MTAP antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 66706-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66706-1-Ig (MTAP antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 66706-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66706-1-Ig (MTAP antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 66706-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66706-1-Ig (MTAP antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 66706-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66706-1-Ig (MTAP antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 66706-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66706-1-Ig (MTAP antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 66706-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66706-1-Ig (MTAP antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat liver using 66706-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 66706-1-Ig (MTAP antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat liver using 66706-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 66706-1-Ig (MTAP antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 66706-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MTAP antibody (66706-1-Ig, Clone: 2B1G6 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: NIH/3T3-Zellen, HCT 116-Zellen, HT-29-Zellen, Raji-Zellen
• Erfolgreiche Detektion in IHC: Mauslebergewebe, humanes Kolonkarzinomgewebe, humanes Leberkarzinomgewebe, humanes Nierengewebe, Mausnierengewebe, Rattenlebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

66706-1-Ig bindet in WB, IHC, IF, ELISA MTAP und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: MTAP fusion protein Ag17954
• Vollständiger Name: methylthioadenosine phosphorylase
• Berechnetes Molekulargewicht: 283 aa, 31 kDa
• Beobachtetes Molekulargewicht: 31 kDa
• GenBank-Zugangsnummer: BC018625
• Gene symbol: MTAP
• Gene ID (NCBI): 4507
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MTAP is a 5-deoxy-5-methylthioadenosine (MTA) phosphorylase, converting MTA to salvageable intermediates including adenine and 5-methylthioribose-1-phosphate. MTAP is abundant in normal cells, but it is frequently found to be deleted in a variety of cancers. Its deficiency is common in cancer cell lines as well as in primary leukemia, lung cancer, melanoma, bladder cancer, gliomas and breast cancer.