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MTAP Rekombinanter Antikörper

MTAP Rekombinant Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

240030A3

Kat-Nr. : 83005-1-PBS

Synonyme

240030A3, 5'-methylthioadenosine phosphorylase, c86fus, EC:2.4.2.28, MSAP

Filter:
  • Western Blot (WB) analysis of HEK-293 cells using MTAP Recombinant antibody (83005-1-RR)

    WB analysis of HEK-293 using 83005-1-RR (same clone as 83005-1-PBS)

    WB result of MTAP antibody (83005-1-RR; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MTAP transfected HEK-293 cells. This data was developed using the same antibody clone with 83005-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using MTAP Recombinant antibody (83005-1-RR)

    WB analysis using 83005-1-RR (same clone as 83005-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83005-1-RR (MTAP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83005-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83005-1-RR

    Affinity and Kinetic Characterization of 83005-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83005-1-RR against Human MTAP were performed. The affinity constant is 0.782 nM.

Geprüfte Anwendungen

Produktinformation

83005-1-PBS bindet in WB, Indirect ELISA MTAP und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen MTAP fusion protein Ag2051
Vollständiger Name methylthioadenosine phosphorylase
Berechnetes Molekulargewicht 283 aa, 31 kDa
Beobachtetes Molekulargewicht31 kDa
GenBank-ZugangsnummerBC018625
Gene symbol MTAP
Gene ID (NCBI) 4507
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MTAP is a 5-deoxy-5-methylthioadenosine (MTA) phosphorylase, converting MTA to salvageable intermediates including adenine and 5-methylthioribose-1-phosphate. MTAP is abundant in normal cells, but it is frequently found to be deleted in a variety of cancers. Its deficiency is common in cancer cell lines as well as in primary leukemia, lung cancer, melanoma, bladder cancer, gliomas and breast cancer.