MTAP Rekombinanter Antikörper

• Featured Product
• KD/KO Validated

MTAP Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

240030A3

Kat-Nr. : 83005-1-RR

Synonyme

240030A3, 5'-methylthioadenosine phosphorylase, c86fus, EC:2.4.2.28, MSAP

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  WB analysis of HEK-293 using 83005-1-RR

  WB result of MTAP antibody (83005-1-RR; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MTAP transfected HEK-293 cells.

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  WB analysis using 83005-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83005-1-RR (MTAP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 83005-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83005-1-RR against Human MTAP were performed. The affinity constant is 0.782 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, C6-Zellen, HCT 116-Zellen, HEK-293-Zellen, HT-29-Zellen, NIH/3T3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83005-1-RR bindet in WB, ELISA MTAP und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: MTAP fusion protein Ag2051
• Vollständiger Name: methylthioadenosine phosphorylase
• Berechnetes Molekulargewicht: 283 aa, 31 kDa
• Beobachtetes Molekulargewicht: 31 kDa
• GenBank-Zugangsnummer: BC018625
• Gene symbol: MTAP
• Gene ID (NCBI): 4507
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MTAP is a 5-deoxy-5-methylthioadenosine (MTA) phosphorylase, converting MTA to salvageable intermediates including adenine and 5-methylthioribose-1-phosphate. MTAP is abundant in normal cells, but it is frequently found to be deleted in a variety of cancers. Its deficiency is common in cancer cell lines as well as in primary leukemia, lung cancer, melanoma, bladder cancer, gliomas and breast cancer.