MTAP Rekombinanter Antikörper

MTAP Rekombinant Antikörper für IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

240030E5

Kat-Nr. : 83005-5-RR

Synonyme

MTA phosphorylase, MSAP, EC:2.4.2.28, c86fus, 5'-methylthioadenosine phosphorylase

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  IF Staining of HeLa using 83005-5-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using MTAP antibody (83005-5-RR, Clone: 240030E5 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1).

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  FC experiment of HeLa using 83005-5-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug MTAP Recombinant antibody (83005-5-RR, Clone:240030E5) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  Affinity and Kinetic Characterization of 83005-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83005-5-RR against Human MTAP were performed. The affinity constant is 1.54 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen
• Erfolgreiche Detektion in FC (Intra): HeLa-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:125-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83005-5-RR bindet in IF/ICC, ELISA MTAP und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: MTAP fusion protein Ag2051
• Vollständiger Name: methylthioadenosine phosphorylase
• Berechnetes Molekulargewicht: 283 aa, 31 kDa
• GenBank-Zugangsnummer: BC018625
• Gene symbol: MTAP
• Gene ID (NCBI): 4507
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MTAP is a 5-deoxy-5-methylthioadenosine (MTA) phosphorylase, converting MTA to salvageable intermediates including adenine and 5-methylthioribose-1-phosphate. MTAP is abundant in normal cells, but it is frequently found to be deleted in a variety of cancers. Its deficiency is common in cancer cell lines as well as in primary leukemia, lung cancer, melanoma, bladder cancer, gliomas and breast cancer.