• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

MTCL2 Polyklonaler Antikörper

MTCL2 Polyklonal Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 33016-1-PBS

Synonyme

80-kDa SOGA fragment, C20orf117, C-terminal 80 kDa form, KIAA0889, Microtubule cross-linking factor 2

Filter:
  • Western Blot (WB) analysis of various lysates using MTCL2 Polyclonal antibody (33016-1-AP)

    WB analysis using 33016-1-AP (same clone as 33016-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 33016-1-AP (C20orf117 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 33016-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using MTCL2 Polyclonal antibody (33016-1-AP)

    WB analysis using 33016-1-AP (same clone as 33016-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 33016-1-AP (C20orf117 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 33016-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

33016-1-PBS bindet in WB, Indirect ELISA MTCL2 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen MTCL2 fusion protein Ag35888
Vollständiger Name chromosome 20 open reading frame 117
Berechnetes Molekulargewicht159kDa
Beobachtetes Molekulargewicht183 kDa, 50 kDa
GenBank-ZugangsnummerNM_080627
Gene symbol C20orf117
Gene ID (NCBI) 140710
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The coiled-coil protein MTCL2 preferentially acts on the perinuclear microtubules accumulated around the Golgi. MTCL2 associates with the Golgi membrane through the N-terminal coiled-coil region and directly binds microtubules through the conserved C-terminal domain without promoting microtubule stabilization. Knockdown of MTCL2 significantly impaired microtubule accumulation around the Golgi, as well as the compactness of the Golgi ribbon assembly structure. MTCL2 forms parallel oligomers through homo-interaction of the central coiled-coil motifs, and promotes asymmetric microtubule organization by crosslinking microtubules on the Golgi membrane. Results of in vitro wound healing assays further suggest that this function of MTCL2 enables integration of the centrosomal and Golgi-associated microtubules on the Golgi membrane, supporting directional migration. Additionally, the results demonstrated the involvement of CLASPs and giantin in mediating the Golgi association of MTCL2 (PMID: 35543016).