MTCO2 Monoklonaler Antikörper

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• KD/KO Validated

MTCO2 Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

CloneNo.

10G14F4

Kat-Nr. : 68527-1-Ig

Synonyme

COX2, 10G14F4, COII, COX 2, COXII

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• 

  WB analysis of 143B.TK.P0 using 68527-1-Ig

  WB result of MTCO2 antibody (MTCO2; 1:1000; incubated at room temperature for 1.5 hours) with 143B and 143B.TK.ρ0 cell. 143B is wild-type cell line, 143B.TK.ρ0 is mtDNA deletion cell line.

• 

  WB analysis using 68527-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68527-1-Ig (MTCO2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: U2OS-Zellen, A431-Zellen, HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, K-562-Zellen, LNCaP-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

68527-1-Ig bindet in WB, ELISA MTCO2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: cytochrome c oxidase II
• Berechnetes Molekulargewicht: 26 kDa
• Beobachtetes Molekulargewicht: 23-26 kDa
• GenBank-Zugangsnummer: YP_003024029
• Gene symbol: MTCO2
• Gene ID (NCBI): 4513
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MTCO2, also named as COII, COXII, and COX2, belongs to the cytochrome c oxidase subunit 2 family. It is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Subunits 1-3 form the functional core of the enzyme complex. Subunit 2 transfers the electrons from cytochrome c via its binuclear copper A center to the bimetallic center of the catalytic subunit 1. Defects in COX2 are a cause of mitochondrial complex IV deficiency (MT-C4D).

Publikationen

Species	Application	Title
human	WB	J Transl Med; DMC-BH derivative DMC-GF inhibits the growth of glioma stem cells by targeting the TRIM33/SLC25A1/mitochondrial oxidative phosphorylation pathway; Authors - Lei Shi